TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam or .75 ml 10% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5 ml TTC (5%) or 7.5 ml TTC (1%)
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 Barrick Lab  >  ProtocolsMediaRecipes  >  ProtocolsRecipesTetrazoliumSugar

Contributors to this topic Edit topic CraigBarnhart, JeffreyBarrick, AlexaMorton, JordanMonk
Topic revision: r1 - 2011-06-14 - 12:43:43 - Main.CraigBarnhart
 
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