ProtocolsRecipesTetrazoliumSugar < Lab

Combine in a 1 L flask:

Add dH₂O to 433.33 mL.

Combine in a 125 ml flask:

500 mL	Component
5 g	Sugar (arabinose = TA, maltose = TM, lactose = TL)

Add dH₂O to 66.67 mL.

Cover each flask with foil, and autoclave sugar and media separately. Total combined volume will be 500 mL.

Combine the autoclaved sugar and media solutions and add:

500 mL	Component
500 µL	5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C

Topic revision: r8 - 2025-01-09 - AlexaMorton