TA: Tetrazolium Sugar (TA, TM, TL, ...)
Combine in a 2 L flask:
| 1.5L |
Component |
| 15 g |
Tryptone |
| 1.5 g |
Yeast Extract |
| 7.5 g |
NaCl |
| 24 g |
Agar |
| 1.5 ml |
5% Antifoam |
Add dH
2O to 1.3 liter.
Combine in a 500 ml flask:
| 1.5L |
Component |
| 6 g |
Sugar (arabinose = TA, maltose = TM, lactose = TL) |
Add dH
2O to 0.2 liter.
Autoclave sugar and media separately.
Total combined volume is 1.5 liters.
Combine and add:
| 1.5L |
Component |
| 1.5 ml |
TTC (5%) |
Source:
Lenski Lab Protocol
Topic revision: r4 - 2012-05-04 - 14:50:29 - Main.JeffreyBarrick
Barrick Lab 
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