(r5) ProtocolsRecipesTetrazoliumSugar < Lab

Tags: view all tags

---+++ TA: Tetrazolium Sugar (TA, TM, TL, ...)


Combine in a 2 L flask:
|  *1.5L*  |  *Component*  |
| 15 g | Tryptone | 
| 1.5 g | Yeast Extract |
| 7.5 g | NaCl | 
| 24 g | Agar | 
| 1.5 ml | 5% Antifoam | 
Add dH<sub>2</sub>O to 1.3 liter.

Combine in a 500 ml flask:
|  *1.5L*  |  *Component*  |
| 15 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) | 
Add dH<sub>2</sub>O to 0.2 liter.

Autoclave sugar and media separately.
Total combined volume is 1.5 liters.

Combine and add:
|  *1.5L*  |  *Component*  |
| 1.5 ml | TTC (5%) |

Source: [[http://myxo.css.msu.edu/ecoli/taagar.html][Lenski Lab Protocol]]

Topic revision: r5 - 2015-03-21 - JordanMonk