(r1) ProtocolsRecipesTetrazoliumSugar < Lab

---+++ TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:
| 15 g | Tryptone | 
| 1.5 g | Yeast Extract |
| 7.5 g | NaCl | 
| 24 g | Agar | 
| 1.5 ml | 5% Antifoam | or | .75 ml | 10% Antifoam |
Add dH<sub>2</sub>O to 1.3 liter.

Combine in a 500 ml flask:
| 6 g | Sugar (arabinose = TA, maltose = TM, lactose = TL) | 
Add dH<sub>2</sub>O to 0.2 liter.

Autoclave sugar and media separately.
Total combined volume is 1.5 liters.

Combine and add:
| 1.5 ml | TTC (5%) | or | 7.5 ml | TTC (1%) |

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesTetrazoliumSugar

Topic revision: r1 - 2011-06-14 - 12:43:43 - Main.CraigBarnhart

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