*Sources:* 1 Datsenko, K.A., and Wanner, B.L. (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. _Proc. Natl. Acad. Sci. U.S.A._ *97*: 6640-6645. 1 Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K.A., Tomita, M., Wanner, B.L., and Mori, H. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 0008. 1 [[http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/Del_construct.html][Keio Strain construction details]]
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