Difference: ProtocolsRecipesTetrazoliumSugar (1 vs. 8)

Revision 82025-01-09 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Changed:
<
<		Combine in a 2 L flask:
1.5L
<-- -->
Sorted ascending 		Component
1.5 g Yeast Extract
1.5 ml 5% Antifoam
7.5 g NaCl
15 g Tryptone
24 g Agar
Add dH2O to 1.3 liter.
>
>		Combine in a 1 L flask:
500 mL Component
5 g Tryptone
500 mg Yeast Extract
2.5 g NaCl
8 g Agar
500 µL 5% Antifoam
Add dH2O to 433.33 mL.
 
Changed:
<
<		Combine in a 500 ml flask:
1.5L Component
15 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.
>
>		Combine in a 125 ml flask:
500 mL Component
5 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 66.67 mL.
 
Changed:
<
<		Autoclave sugar and media separately. Total combined volume is 1.5 liters.
>
>		Cover each flask with foil, and autoclave sugar and media separately. Total combined volume will be 500 mL.
 
Changed:
<
<		Combine and add:
1.5L Component
1.5 ml 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
>
>		Combine the autoclaved sugar and media solutions and add:
500 mL Component
500 µL 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
  Source: Lenski Lab Protocol

Revision 72019-03-08 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L Component
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

1.5L Component
15 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L Component
Changed:
<
<
1.5 ml 5% (w/v) Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
>
>
1.5 ml 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
  Source: Lenski Lab Protocol

Revision 62018-10-25 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L Component
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

1.5L Component
15 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L Component
Changed:
<
<
1.5 ml TTC (5%)
>
>
1.5 ml 5% (w/v) Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
  Source: Lenski Lab Protocol

Revision 52015-03-21 - JordanMonk

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L Component
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

1.5L Component
Changed:
<
<
6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
>
>
15 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
 Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L Component
1.5 ml TTC (5%)

Source: Lenski Lab Protocol

Revision 42012-05-04 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L Component
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

1.5L Component
6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L Component
Changed:
<
<
1.5 ml TTC (5%) or 7.5 ml TTC (1%)
>
>
1.5 ml TTC (5%)
Added:
>
>		 Source: Lenski Lab Protocol
 

Revision 32011-07-29 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Added:
>
>
 Combine in a 2 L flask:
Added:
>
>
1.5L Component
 
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

Added:
>
>
1.5L Component
 
6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

Added:
>
>
1.5L Component
 
1.5 ml TTC (5%) or 7.5 ml TTC (1%)

Revision 22011-07-26 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
Changed:
<
<
1.5 ml 5% Antifoam or .75 ml 10% Antifoam
>
>
1.5 ml 5% Antifoam
 Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5 ml TTC (5%) or 7.5 ml TTC (1%)

Revision 12011-06-14 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam or .75 ml 10% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5 ml TTC (5%) or 7.5 ml TTC (1%)
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