(r1) ProtocolsRecipesSOB < Lab

SOB: Super Optimal Broth

1L	Final [ ]	Component
5 g	0.5%	yeast extract
20 g	2.0%	tryptone
0.5 g	10 mM	NaCl
0.186 g	2 mM	KCl
2.4 g	20 mM	MgSO₄

Add 800 ml of water and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add H₂O to a final volume of 960 ml. Autoclave.

Barrick Lab > ProtocolsMediaRecipes > ProtocolsRecipesSOB

Topic revision: r1 - 2012-01-11 - 18:12:27 - Main.CraigBarnhart

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