LB Agar: Lysogeny Broth

Combine in a 2 L flask the following:

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

• Common synonyms are Luria-Broth and Luria-Bertani medium.
• This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter!*
• If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
• To make "soft" agar for phage studies, use only 7 g agar per liter.