ProtocolsRecipesDR < Lab

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---+++ DR:  Defined minimal media for D. radiodurans

|  *250 ml*  |   *500 ml*  |  *Component*  |
| 50 ml | 100 ml | 5x M9 salts | 
| 250 ul | 500 ul | 5mM MnCl<sub>2 |
| 250 ul | 500 ul | 0.8M MgCl<sub>2 |
| 250 ul | 500 ul | 0.18 M CaCl<sub>2 |
| 2.5 ml | 5 ml | Syringe filtered Vitamins Mix |
| 500 ul | 1 ml | Syringe filtered Amino Acid mix (see below) |
Add dH<sub>2</sub>O to final volume. <br>
Note:  All components are either pre-sterilized or syringe filtered so the only things that need to be autoclaved are the receiving bottle and the added water.


To prepare the amino acids: <br>
| *Amount* | *Amino Acid* |
| 1 g | L-Cys |
| 0.5 g | L-His |
| 0.5 g | L-Met |
Dissolve these amino acids in 40 ml of dH<sub>2</sub>O

Topic revision: r1 - 2011-07-29 - CraigBarnhart