ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

To make standard ABMS, combine the following pre-sterilized ingredients at room temperature:

To make ABMS agar:

Per liter of media, add 16 g/L agar and 1 mL/L antifoam to diH₂O, then autoclave in a 2 L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55°C in a water bath before combining with agar to reduce the temperature difference when combined.

ABMS Component Recipes

SL9 (trace minerals)

Add 1 mL of 10 mL 10× solution:

Adjust pH to 6.5 using 1 M NaOH. Bring volume to 1000 mL with diH₂O. Note: this requires a lot of NaOH, so leave room when bringing up to volume.

Mineral Solution (20×)

† Substitution: You may use 465.8 µL of a 1 M CaCl₂ per 500 ml of stock solution for this component. The final concentration of Ca²⁺ in the 20× stock is 9.12 mM.

Bring to 500mL in a graduated cylinder with deionized water.

Phosphate Buffer (20×)

Bring to 500 mL with diH₂O; pH should be ~6.9-7.0 (you may add 400 mL diH₂O first and bring pH up to 6.8 then complete volume to 500mL)

* Source: Averhoff lab