(r1) Barrick Lab :: ProcedureEvolvabilityMutationAccumulation

---++ Day 1: Plating the Mixed Population

   1. Find microsatellite-containing strains in the -80°C freezer. They will be labeled with the appropriate microsatellite sequence located in the rhamnose operon (eg. "(CA)<sub>12</sub>"). See below for the list of microsatellites.

---++ Day 2: Picking Colonies

---++ Day 3: Passaging Colonies

| *Number* | *Sequence* | *Position* | *Fluorescent Tag?* | 
| 1 | (CA)<sub>6</sub> | _rhaT-rhaD_ | |
| 2 | (CA)<sub>12</sub> | _rhaT-rhaD_ | |
| 3 | (TA)<sub>12</sub> | _rhaT-rhaD_ | |
| 4 | (A)<sub>24</sub> | _rhaT-rhaD_ | |
| 5 | (AGA)<sub>8</sub> | _rhaT-rhaD_ | |
| 6 | (GGA)<sub>8</sub> | _rhaT-rhaD_ | |
| 7 | (G)<sub>9</sub> | _rhaT-rhaD_ | |
| 8 | (G)<sub>12</sub> | _rhaT-rhaD_ | |








-- Main.LindseyWolf - 18 May 2011

Barrick Lab > ProtocolList > ProcedureEvolvabilityMutationAccumulation

Contributors to this topic

LindseyWolf

Topic revision: r1 - 2011-05-18 - 19:15:10 - Main.LindseyWolf