Difference: ProtocolsRecipesM9Minimal (1 vs. 8)

Revision 82018-11-14 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

Changed:

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<

M9 Minimal Media

>
>

M9 Minimal Medium

1L	Component
6 g	Sodium phosphate dibasic (anhydrous), Na₂HPO₄
3 g	Potassium phosphate monobasic, KH₂PO₄
0.5 g	Sodium chloride, NaCl
1 g	Ammonium chloride, NH₄Cl

Add dH₂O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:

1L	Component
1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂
10 ml	10% (w/v) glucose (or other carbon source)

Final composition
93.0 mM	Sodium (Na⁺)
22.1 mM	Potassium (K⁺)
18.7 mM	Ammonium (NH₄)
1.0 mM	Calcium (Ca²⁺)
0.1 mM	Magnesium (Mg²⁺)
29.2 mM	Chloride (Cl^-
0.1 mM	Sulfate (SO₄^2-)
42.2 mM	Phosphate (PO₄^3-)

Revision 72015-09-20 - JordanMonk

META TOPICPARENT	name="ProtocolsMediaRecipes"

M9 Minimal Media

1L	Component

Changed:

<
<

6 g	Sodium phosphate, Na₂HPO₄
3 g	Potassium phosphate, KH₂PO₄
0.5 g	Sodium chloride, NaCl

>
>

6 g	Sodium phosphate dibasic (anhydrous), Na₂HPO₄
3 g	Potassium phosphate monobasic, KH₂PO₄
0.5 g	Sodium chloride, NaCl

1 g	Ammonium chloride, NH₄Cl

Add dH₂O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:

1L	Component

Changed:

<
<

1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂

>
>

1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂

10 ml

10% (w/v) glucose (or other carbon source)

Final composition
93.0 mM	Sodium (Na⁺)
22.1 mM	Potassium (K⁺)
18.7 mM	Ammonium (NH₄)
1.0 mM	Calcium (Ca²⁺)
0.1 mM	Magnesium (Mg²⁺)
29.2 mM	Chloride (Cl^-
0.1 mM	Sulfate (SO₄^2-)
42.2 mM	Phosphate (PO₄^3-)

Revision 62013-06-21 - AurkoDasgupta

META TOPICPARENT	name="ProtocolsMediaRecipes"

M9 Minimal Media

1L	Component
6 g	Sodium phosphate, Na₂HPO₄
3 g	Potassium phosphate, KH₂PO₄
0.5 g	Sodium chloride, NaCl
1 g	Ammonium chloride, NH₄Cl

Add dH₂O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:

1L	Component
1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂
10 ml	10% (w/v) glucose (or other carbon source)

Added:

>
>

Final composition
93.0 mM	Sodium (Na⁺)
22.1 mM	Potassium (K⁺)
18.7 mM	Ammonium (NH₄)
1.0 mM	Calcium (Ca²⁺)
0.1 mM	Magnesium (Mg²⁺)
29.2 mM	Chloride (Cl^-
0.1 mM	Sulfate (SO₄^2-)
42.2 mM	Phosphate (PO₄^3-)

Revision 52012-06-04 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

Changed:

<
<

M9 Minimal

>
>

M9 Minimal Media

Deleted:

<
<

Make 5× M9 salts:

1L	Component

Changed:

<
<

64 g	Sodium phosphate (dibasic, 7 hydrate) Na₂HPO₄•H₂O
15 g	Potassium phosphate (monobasic) KH₂PO₄
2.5 g	Sodium chloride, NaCl
5 g	Ammonium chloride NH₄Cl

>
>

6 g	Sodium phosphate, Na₂HPO₄
3 g	Potassium phosphate, KH₂PO₄
0.5 g	Sodium chloride, NaCl
1 g	Ammonium chloride, NH₄Cl

Add dH₂O to 1 liter. Autoclave.

Changed:

<
<

Combine the following ingredients (all pre-sterilized):

>
>

After media is autoclaved add the following sterile ingredients:

1L	Component

Changed:

<
<

200 ml	5× M9 salts
2 ml	1M Magnesium sulfate MgSO₄
0.1 ml	1 M Calcium chloride CaCl₂

>
>

1 ml	1M Magnesium sulfate, MgSO₄
1 ml	0.1M Calcium chloride CaCl₂
10 ml	10% (w/v) glucose (or other carbon source)

Deleted:

<
<

40 ml

10% (w/v) glucose (or other carbon source)

Add sterile dH₂O to 1 liter. Autoclave.

To make M9 plates add the items above to 16g of sterilized agar.
If making less than 1 liter use 8g of agar per 500ml of solution.

Revision 42011-07-29 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

M9 Minimal

Make 5× M9 salts:

Added:

>
>

1L	Component

64 g	Sodium phosphate (dibasic, 7 hydrate) Na₂HPO₄•H₂O
15 g	Potassium phosphate (monobasic) KH₂PO₄
2.5 g	Sodium chloride, NaCl
5 g	Ammonium chloride NH₄Cl

Add dH₂O to 1 liter. Autoclave.

Combine the following ingredients (all pre-sterilized):

Added:

>
>

1L	Component

200 ml	5× M9 salts
2 ml	1M Magnesium sulfate MgSO₄
0.1 ml	1 M Calcium chloride CaCl₂
40 ml	10% (w/v) glucose (or other carbon source)

Add sterile dH₂O to 1 liter. Autoclave.

To make M9 plates add the items above to 16g of sterilized agar.
If making less than 1 liter use 8g of agar per 500ml of solution.

Revision 32011-06-27 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

M9 Minimal

Make 5× M9 salts:

64 g	Sodium phosphate (dibasic, 7 hydrate) Na₂HPO₄•H₂O
15 g	Potassium phosphate (monobasic) KH₂PO₄
2.5 g	Sodium chloride, NaCl
5 g	Ammonium chloride NH₄Cl

Add dH₂O to 1 liter. Autoclave.

Combine the following ingredients (all pre-sterilized):

200 ml	5× M9 salts
2 ml	1M Magnesium sulfate MgSO₄
0.1 ml	1 M Calcium chloride CaCl₂
40 ml	10% (w/v) glucose (or other carbon source)

Changed:

<
<

Add sterile dH₂O to 1 liter. Autoclave. |

>
>

Add sterile dH₂O to 1 liter. Autoclave.

Changed:

<
<

To make M9 plates add the items above to 16g of sterilized agar.

>
>

To make M9 plates add the items above to 16g of sterilized agar.

Added:

>
>

If making less than 1 liter use 8g of agar per 500ml of solution.

Revision 22011-06-23 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

M9 Minimal

Make 5× M9 salts:

64 g	Sodium phosphate (dibasic, 7 hydrate) Na₂HPO₄•H₂O
15 g	Potassium phosphate (monobasic) KH₂PO₄
2.5 g	Sodium chloride, NaCl
5 g	Ammonium chloride NH₄Cl

Add dH₂O to 1 liter. Autoclave.

Combine the following ingredients (all pre-sterilized):

200 ml	5× M9 salts
2 ml	1M Magnesium sulfate MgSO₄
0.1 ml	1 M Calcium chloride CaCl₂
40 ml	10% (w/v) glucose (or other carbon source)

Changed:

<
<

Add sterile dH₂O to 1 liter. Autoclave.

>
>

Add sterile dH₂O to 1 liter. Autoclave. |

Added:

>
>

To make M9 plates add the items above to 16g of sterilized agar.

Revision 12011-06-14 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

M9 Minimal

Make 5× M9 salts:

64 g	Sodium phosphate (dibasic, 7 hydrate) Na₂HPO₄•H₂O
15 g	Potassium phosphate (monobasic) KH₂PO₄
2.5 g	Sodium chloride, NaCl
5 g	Ammonium chloride NH₄Cl

Add dH₂O to 1 liter. Autoclave.

Combine the following ingredients (all pre-sterilized):

200 ml	5× M9 salts
2 ml	1M Magnesium sulfate MgSO₄
0.1 ml	1 M Calcium chloride CaCl₂
40 ml	10% (w/v) glucose (or other carbon source)

Add sterile dH₂O to 1 liter. Autoclave.

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