Difference: ProtocolsRecipesLysogenyBrothAgar (1 vs. 11)

Revision 112025-02-19 - TylerDeJong

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Add dH₂O to full volume in an appropriately-sized flask. Combine the following, making sure each component fully dissolves before adding the next:

1L	6L	Component
10 g	60 g	Tryptone
5 g	30 g	Yeast Extract
10 g	60 g	NaCl
1 ml	6 ml	5% Antifoam*
16 g	48 g	Agar*

ANTIFOAM: Open the bottle under sterile conditions (near a flame) only, and continue agitating after the addition of the 5% antifoam.

AGAR: Measure 8 grams of agar per 500 ml bottle and add to each individual bottle before aliquoting the LB. Autoclave to homogenize.

Each liter of LB Agar makes 2 bottles of 500 ml.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.

Changed:

<
<

If you are performing sacB counter-selection, you do not want to include NaCl in your agar. Use the above recipe, but swap out 5-10% w/v (50-100g per liter) sucrose in place of any NaCl. Sucrose can be autoclaved (no need to filter sterilize it separately).

>
>

If you are performing sacB counter-selection, you do not want to include NaCl in your agar. Use the above recipe, but swap out 5-10% w/v (50-100g per liter) sucrose in place of any NaCl. Sucrose can be directly combined with the other components and autoclaved (no need to filter sterilize it separately).

To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

Revision 102025-02-18 - TylerDeJong

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Add dH₂O to full volume in an appropriately-sized flask. Combine the following, making sure each component fully dissolves before adding the next:

1L	6L	Component
10 g	60 g	Tryptone
5 g	30 g	Yeast Extract
10 g	60 g	NaCl
1 ml	6 ml	5% Antifoam*
16 g	48 g	Agar*

ANTIFOAM: Open the bottle under sterile conditions (near a flame) only, and continue agitating after the addition of the 5% antifoam.

AGAR: Measure 8 grams of agar per 500 ml bottle and add to each individual bottle before aliquoting the LB. Autoclave to homogenize.

Each liter of LB Agar makes 2 bottles of 500 ml.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.

Changed:

<
<

If you are performing sacB counter-selection, you may need to use even lower salt concentrations.

>
>

If you are performing sacB counter-selection, you do not want to include NaCl in your agar. Use the above recipe, but swap out 5-10% w/v (50-100g per liter) sucrose in place of any NaCl. Sucrose can be autoclaved (no need to filter sterilize it separately).

To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

Revision 92024-09-05 - SophiaLin

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Changed:

<
<

Combine in a 2 L flask the following:

>
>

Add dH₂O to full volume in an appropriately-sized flask. Combine the following, making sure each component fully dissolves before adding the next:

Changed:

<
<

1.5L <-- -->	Component
1.5 ml	5% Antifoam
7.5 g	Yeast Extract
15 g	Tryptone
15 g	NaCl
24 g	Agar

>
>

1L	6L	Component
10 g	60 g	Tryptone
5 g	30 g	Yeast Extract
10 g	60 g	NaCl
1 ml	6 ml	5% Antifoam*
16 g	48 g	Agar*

Changed:

<
<

Add dH₂O to 1.5 L. Autoclave.

>
>

ANTIFOAM: Open the bottle under sterile conditions (near a flame) only, and continue agitating after the addition of the 5% antifoam.

Added:

>
>

AGAR: Measure 8 grams of agar per 500 ml bottle and add to each individual bottle before aliquoting the LB. Autoclave to homogenize.

Each liter of LB Agar makes 2 bottles of 500 ml.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

Revision 82020-02-11 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

1.5L	Component
15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
If you are performing sacB counter-selection, you may need to use even lower salt concentrations.

Changed:

<
<

To make "soft" agar for phage studies, use only 7 g agar per liter.

>
>

To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

Revision 72019-06-06 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

1.5L	Component
15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.

Changed:

<
<

This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter! They are NOT interchangeable.

>
>

This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.

If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
To make "soft" agar for phage studies, use only 7 g agar per liter.

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

Revision 62015-06-23 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

Changed:

<
<

LB Agar: Lysogeny Broth

>
>

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

1.5L	Component
15 g	Tryptone
7.5 g	Yeast Extract

Changed:

<
<

15 g

NaCl

>
>

15 g

NaCl

24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.

Changed:

<
<

This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter!*

>
>

This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter! They are NOT interchangeable.

If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
To make "soft" agar for phage studies, use only 7 g agar per liter.

Added:

>
>

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

Revision 52013-09-03 - AlvaroRodriguez

META TOPICPARENT	name="ProtocolsMediaRecipes"

Changed:

<
<

LB: Lysogeny Broth

>
>

LB Agar: Lysogeny Broth

Combine in a 2 L flask the following:

1.5L	Component
15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter!*
If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
To make "soft" agar for phage studies, use only 7 g agar per liter.

Revision 42012-11-23 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

Changed:

<
<

LB: Luria-Broth

>
>

LB: Lysogeny Broth

Combine in a 2 L flask the following:

1.5L	Component
15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Changed:

<
<

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

>
>

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter!*

Added:

>
>

If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
To make "soft" agar for phage studies, use only 7 g agar per liter.

Revision 32011-07-29 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Luria-Broth

Changed:

<
<

Combine in a 2 L flask:

>
>

Combine in a 2 L flask the following:

Added:

>
>

1.5L	Component

15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

Revision 22011-07-26 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Luria-Broth

Combine in a 2 L flask:

15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar

Changed:

<
<

1.5 ml

5% Antifoam

.75 ml

10% Antifoam

>
>

1.5 ml

5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

Revision 12011-06-14 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Luria-Broth

Combine in a 2 L flask:

15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam	or	.75 ml	10% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

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