Difference: ReferenceList (1 vs. 58)

Revision 582025-03-20 - IraZibbu

 
META TOPICPARENT name="WebHome"
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Barrick Lab :: Reference Information

Getting Started in Lab

Templates and Spreadsheets

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Experimental Design and Statistics

Bacteria

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General Setup and Getting Started Guides

  • Computer Setup
    How to get your computer ready for bioinformatics and computational biology.
  • Computing Environment Setup
    Advice for setting up your Linux/Unix environment for bioinformatics and computational biology.
  • Learn Biocomputing
    Resources for learning the command line, R, Python, GitHub, etc.
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Revision 572024-07-10 - JeffreyBarrick

 
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  • Computer Setup
    How to get your computer ready for bioinformatics and computational biology.
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    Advice for setting up your Linux/Unix environment for bioinformatics and computational biology.
  • Learn Biocomputing
    Resources for learning the command line, R, Python, GitHub, etc.

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  • Learn Biocomputing
    Resources for learning the command line, R, Python, GitHub, etc.
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Memorable and humorous sayings related to our research.
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Revision 552024-07-09 - PraneshRao

 
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Revision 542024-07-09 - JeffreyBarrick

 
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Revision 532023-10-06 - JeffreyBarrick

 
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Revision 522023-09-21 - JeffreyBarrick

 
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Revision 502020-07-15 - JeffreyBarrick

 
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Revision 492018-11-28 - JeffreyBarrick

 
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Revision 482018-11-12 - SimonDAlton

 
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Revision 432016-12-10 - GabrielSuarez

 
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Revision 422016-07-18 - SeanLeonard

 
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Revision 402014-05-30 - JeffreyBarrick

 
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Revision 372012-05-01 - JeffreyBarrick

 
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Revision 362011-05-17 - JeffreyBarrick

 
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Revision 322011-04-03 - JeffreyBarrick

 
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Revision 292011-03-25 - MichaelHammerling

 
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Long-Term Evolution Experiment

Arabinose Marker

REL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acid are also known to confer an Ara+ phenotype.

Strain Phenotype Color araA allele
REL606 Ara- Red led Red 92D (GAC)
REL607 Ara+ Gray led White 92G (GGC)
other revertants Ara+ Gray led White 92A (GCC)

Color of colonies when grown on tetrazolium arabinose (TA) plates.

Summary of the Known Idiosyncrasies of Each Long-Term Line

After more than 20 years and 40,000 generations of evolution in the laboratory, the various experimental replicates of the long-term experiment have diverged markedly. Some fail to grow on certain media.

Line Growth Other Notes
Red led Ara-1 Normal  
Red led Ara-2 >20k some colonies fail to grow on MA/MG/TA.
Normal growth on LB.
Stable Polymorphism
Red led Ara-3 Normal  
Red led Ara-4 Normal  
Red led Ara-5 Normal  
Red led Ara-6 Normal  
Gray led Ara+1 Normal  
Gray led Ara+2 Normal  
Gray led Ara+3 Normal  
Gray led Ara+4 Normal  
Gray led Ara+5 Normal  
Gray led Ara+6 >=10k unreliable growth on TA, no growth on MA  

Key to Media: minimal arabinose (MA), minimal glucose (MG), tetrazolium arabinose (TA).

 

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Warning!
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Long-Term Evolution Experiment

Arabinose Marker

REL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acid are also known to confer an Ara+ phenotype.

Strain Phenotype Color araA allele
REL606 Ara- Red led Red 92D (GAC)
REL607 Ara+ Gray led White 92G (GGC)
other revertants Ara+ Gray led White 92A (GCC)

Color of colonies when grown on tetrazolium arabinose (TA) plates.

Summary of the Known Idiosyncrasies of Each Long-Term Line

After more than 20 years and 40,000 generations of evolution in the laboratory, the various experimental replicates of the long-term experiment have diverged markedly. Some fail to grow on certain media.

Line Growth Other Notes
Red led Ara-1 Normal  
Red led Ara-2 >20k some colonies fail to grow on MA/MG/TA.
Normal growth on LB.
Stable Polymorphism
Red led Ara-3 Normal  
Red led Ara-4 Normal  
Red led Ara-5 Normal  
Red led Ara-6 Normal  
Gray led Ara+1 Normal  
Gray led Ara+2 Normal  
Gray led Ara+3 Normal  
Gray led Ara+4 Normal  
Gray led Ara+5 Normal  
Gray led Ara+6 >=10k unreliable growth on TA, no growth on MA  

Key to Media: minimal arabinose (MA), minimal glucose (MG), tetrazolium arabinose (TA).

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Long-Term Evolution Experiment

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Arabinose Marker

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Arabinose Marker

  REL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acid are also known to confer an Ara+ phenotype.

Strain Phenotype Color araA allele
REL606 Ara- Red led Red 92D (GAC)
REL607 Ara+ Gray led White 92G (GGC)
other revertants Ara+ Gray led White 92A (GCC)

Color of colonies when grown on tetrazolium arabinose (TA) plates.

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Summary of the Known Idiosyncrasies of Each Long-Term Line

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Summary of the Known Idiosyncrasies of Each Long-Term Line

  After more than 20 years and 40,000 generations of evolution in the laboratory, the various experimental replicates of the long-term experiment have diverged markedly. Some fail to grow on certain media.

Line Growth Other Notes
Red led Ara-1 Normal  
Red led Ara-2 >20k some colonies fail to grow on MA/MG/TA.
Normal growth on LB.
Stable Polymorphism
Red led Ara-3 Normal  
Red led Ara-4 Normal  
Red led Ara-5 Normal  
Red led Ara-6 Normal  
Gray led Ara+1 Normal  
Gray led Ara+2 Normal  
Gray led Ara+3 Normal  
Gray led Ara+4 Normal  
Gray led Ara+5 Normal  
Gray led Ara+6 >=10k unreliable growth on TA, no growth on MA  

Key to Media: minimal arabinose (MA), minimal glucose (MG), tetrazolium arabinose (TA).

Revision 32008-04-27 - JeffreyBarrick

 
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Long-Term Evolution Experiment

Arabinose Marker

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REL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acids may also occur and confer an Ara+ phenotype.
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REL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acid are also known to confer an Ara+ phenotype.
 
Strain Phenotype Color araA allele
REL606 Ara- Red led Red 92D (GAC)
REL607 Ara+ Gray led White 92G (GGC)
other revertants Ara+ Gray led White 92A (GCC)

Color of colonies when grown on tetrazolium arabinose (TA) plates.

Added:
>
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Summary of the Known Idiosyncrasies of Each Long-Term Line

After more than 20 years and 40,000 generations of evolution in the laboratory, the various experimental replicates of the long-term experiment have diverged markedly. Some fail to grow on certain media.

Line Growth Other Notes
Red led Ara-1 Normal  
Red led Ara-2 >20k some colonies fail to grow on MA/MG/TA.
Normal growth on LB.
Stable Polymorphism
Red led Ara-3 Normal  
Red led Ara-4 Normal  
Red led Ara-5 Normal  
Red led Ara-6 Normal  
Gray led Ara+1 Normal  
Gray led Ara+2 Normal  
Gray led Ara+3 Normal  
Gray led Ara+4 Normal  
Gray led Ara+5 Normal  
Gray led Ara+6 >=10k unreliable growth on TA, no growth on MA  

Key to Media: minimal arabinose (MA), minimal glucose (MG), tetrazolium arabinose (TA).

 

Revision 22008-04-27 - JeffreyBarrick

 
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Long-Term Evolution Experiment

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REL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acids may also occur and confer an Ara+ phenotype.

Strain Phenotype Color araA allele
REL606 Ara- Red led Red 92D (GAC)
REL607 Ara+ Gray led White 92G (GGC)
other revertants Ara+ Gray led White 92A (GCC)

Color of colonies when grown on tetrazolium arabinose (TA) plates.

 

Revision 12008-04-26 - JeffreyBarrick

 
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Long-Term Evolution Experiment

  • Arabinose Marker
 
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