Commonly Used Plasmids

plasmid markers origin host ref description
pCA24N CamR     Kitagawa2005 ASKA collection vector
pKD46 AmpR TS ? Datsenko&Wanner2000 Wanner lambda red plasmid, temperature sensitive origin grow at 30C! Low copy number.
pKD78 CamR TS     Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3 CamR   BW25141 Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   BW25141 Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR       TOPO cloning kit vector
pACYC184 TetR, CamR   ER2420 Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR     Link1997 Church vectors for creating deletions; note: Grow at 30 Celsius
pACBSR CamR p15A   Herring2003 Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302 SpecR     Akakura2002 Broad host range, promoterless lacZ map
pCP20 AmpR TS BT340 FLP recombinase protocol Baba 2006 Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection.

Plasmids listed on this page reside in the common plasmid box in the 80C freezers in both Welch and MBB labs.

Useful Links

Topic attachments
I Attachment Action Size Date Who Comment
elsegbk pACYC184.gbk manage 7.7 K 03 Aug 2009 - 21:09 JeffreyBarrick  
elsegbk pCA24N.gbk manage 9.9 K 29 May 2009 - 13:21 JeffreyBarrick  
elsegbk pCR2.1.gbk manage 7.1 K 07 Mar 2012 - 21:11 JeffreyBarrick  
elsegbk pKD13_Kan_template.gbk manage 7.6 K 29 May 2009 - 13:22 JeffreyBarrick  
elsegbk pKD3_Cam_template.gbk manage 6.6 K 29 May 2009 - 13:22 JeffreyBarrick  
elsegb pKD46.gb manage 16.4 K 20 Jan 2012 - 21:09 BrianRenda  
txttxt pKOV.txt manage 10.6 K 21 Feb 2011 - 17:13 JeffreyBarrick  
elsegbk pkd4.gbk manage 6.1 K 19 Dec 2011 - 22:29 BrianRenda  
elsegbk pkd4_with_gfp_sites_marked.gbk manage 10.0 K 19 Dec 2011 - 22:35 BrianRenda  

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Topic revision: r22 - 12 May 2014 - 16:03:13 - DanielDeatherage
 
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