Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,544,3520T→A12.0% 1.5 ‑14.4 0.06789xasApredicted glutamate:gamma‑aminobutyric acid antiporter
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/11):  ref base (45/11):  total (45/22)
Fisher's exact test strand distribution p-value = 5.49e-07
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

ATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAG  >  REL606/1544317‑1544387
                                   |                                   
aTGAAAAGCCCGATGTTGCGAAGGTAGGGTCTTCAt                                     <  1:5534617/36‑1 (MQ=255)
aTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCAt                                     <  1:3811205/36‑1 (MQ=255)
aTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCAt                                     <  1:1516234/36‑1 (MQ=255)
aTGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCAt                                     <  1:2613586/36‑1 (MQ=255)
 tGAAAAGCCCGCTGTTGCGAAGGTAGGGTATTCATa                                    >  1:7624981/1‑36 (MQ=255)
 tGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATa                                    >  1:862975/1‑36 (MQ=255)
 tGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATa                                    >  1:5569898/1‑36 (MQ=255)
  gAAAAGCCCGCTGTTTCGAAGGTAGGGTATTCATaa                                   <  1:4163151/36‑1 (MQ=37)
  gAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATaa                                   >  1:7109437/1‑36 (MQ=255)
  gAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATaa                                   >  1:6463427/1‑36 (MQ=255)
  gAAAAGCCAGATGTTGCGAAGGTAGGGTATTCATaa                                   >  1:3254016/1‑36 (MQ=255)
   aaaaGCCCGATGTTGCGAAGGTAGGGTATTCATaaa                                  >  1:6447316/1‑36 (MQ=255)
   aaaaGCCCGATGTTGCGAAGGTAGGGTATTCATaaa                                  <  1:4674874/36‑1 (MQ=255)
   aaaaGCCCGATGTTGCGAAGGTAGGGTATTCATaaa                                  >  1:2677110/1‑36 (MQ=255)
    aaaGCCCGATGTTGCGAAGGTAGGGTATTCATAAAc                                 <  1:2703196/36‑1 (MQ=255)
    aaaGCCCGATGTTGCGAAGGTAGGGTATTCATAAAc                                 <  1:90688/36‑1 (MQ=255)
     aaGCCCGATGTTGCGAAGGTAGGGTATTCATAAACa                                >  1:76876/1‑36 (MQ=255)
      aGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAg                               >  1:5382661/1‑36 (MQ=255)
       gCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGc                              <  1:757606/36‑1 (MQ=255)
       gCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGc                              <  1:3030004/36‑1 (MQ=255)
        ccggATGTTGCGAAGGTAGGGTATTCATAAACAGcc                             <  1:2400477/33‑1 (MQ=255)
        cccGATGTTGCGAAGGTAGGGTATTCATAAACAGcc                             <  1:4978605/36‑1 (MQ=255)
         ccGATGTTGCGAAGGTAGGGTATTCATAAACAGCCa                            >  1:5092364/1‑36 (MQ=255)
         ccGATGTTGCGAAGGTAGGGTATTCATAAACAGCCa                            >  1:4095182/1‑36 (MQ=255)
          cGATGTTGCGAAGGTAGGGTATTCATAAACAGCCAt                           >  1:1096187/1‑36 (MQ=255)
           gATGTTGCGAAGGTAGGGTATTCATAAACAGCCAtt                          >  1:6210765/1‑36 (MQ=255)
            aTGTTGCGAAGGTAGGGTATTCATAAACAGCCATTa                         >  1:5859324/1‑36 (MQ=255)
            aTGTTGCGAAGGTAGGGTATTCATAAACAGCCATTa                         >  1:2232818/1‑36 (MQ=255)
              gTTGCGAAGGTAGGGTATTCAAAAACAGCCATTAcc                       <  1:6627704/36‑1 (MQ=255)
               ttGCGAAGGTAGGGTATTCATAAACAGCCATTACCa                      >  1:5011275/1‑36 (MQ=255)
               ttGCGAAGGCAGGGTATTCATAAACAGCCATTACCa                      >  1:3275148/1‑36 (MQ=255)
               cggCGAAGGTAGGGTATTCATAAACAGCCATTACCa                      >  1:7315721/3‑36 (MQ=255)
                tGCGAAGGTAGGGTATTCATAAACAGCCATTACCAt                     >  1:118081/1‑36 (MQ=255)
                tGCGAAGGTAGGGTATTCATAAACAGCCATTACCAt                     >  1:2128939/1‑36 (MQ=255)
                tGCGAAGGTAGGGTATTCATAAACAGCCATTACCAt                     >  1:255619/1‑36 (MQ=255)
                 gggAAGGTAGGGTATTAATAACCAGCCATTACCATc                    <  1:3901637/34‑1 (MQ=37)
                 gaaaaGGTAGTTTATCAAAAAACAGCCATTACCATc                    <  1:3906414/33‑1 (MQ=9)
                 gCGAAGGTAGGGTATTCAAAAACAGCCATTACCATc                    <  1:6302080/36‑1 (MQ=255)
                 gCGAAGGTAGGGTATTAATAAACAGCCATTACCATc                    <  1:1602002/36‑1 (MQ=255)
                  ggAAGGTAGGGTATTCAAAAACAGCCATTACCATCg                   <  1:7391528/35‑1 (MQ=255)
                   gAAGGTAGGGTATTCATAAACAGCCATTACCATCGa                  >  1:1919803/1‑36 (MQ=255)
                   gAAGGTAGGGTATTCATAAACAGCCATTACCATCGa                  >  1:2232484/1‑36 (MQ=255)
                   gAAGGTAGGGTATTCATAAACAGCCATTACCATCGa                  >  1:4301880/1‑36 (MQ=255)
                    agggTAGGTTATTAAAAAACAGCCATTACCATCGAt                 <  1:734034/34‑1 (MQ=21)
                    agggTAGGGTATTAAAAAACAGCCATTACCATCGAt                 <  1:6618426/34‑1 (MQ=37)
                    aaGGTAGGTTATTCAAAAACAGCCATTACCATCGAt                 <  1:4206098/36‑1 (MQ=37)
                    aaGGTAGGGTATTCATAAACAGCCATTACCATCGAt                 >  1:6696690/1‑36 (MQ=255)
                     aGGTAGGTTATAAATAAACAGCCATTACCATCGATg                <  1:7392788/36‑1 (MQ=21)
                      ggTAGGGTATTCATAAACAGCCATTACCATCGATGc               >  1:7814873/1‑36 (MQ=255)
                       ttAGGTTATTAAAAAACAGCCATTACCATCGATGcc              <  1:3025172/35‑1 (MQ=25)
                       ggaGGTTATTAAAAAACAGCCATTACCATCGATGcc              <  1:1313123/34‑1 (MQ=21)
                       gTAGGGTATTCATAAACAGCCATTACTATCGCTGcc              >  1:2483786/1‑36 (MQ=37)
                        tAGGGTATTCATAAACAGCCATTACCATCGATGCCg             >  1:3837399/1‑36 (MQ=255)
                         aGGGTATTAAAAAACAGCCATTACCATCGATGCCGt            <  1:5948551/36‑1 (MQ=37)
                         aGGGTATACAAAAACAGCCATTACCATCGATGCCGt            <  1:7335230/36‑1 (MQ=37)
                          gggTATTCATAAACAGCCATTACCATCGATGCCGtt           >  1:4229689/1‑36 (MQ=255)
                           ggTATTCATAAACAGCCATTACCATCGATGCCGTTa          >  1:5714281/1‑36 (MQ=255)
                            gTATTCATAAACAGCCATTACCATCGATGCCGTTAt         >  1:6295799/1‑36 (MQ=255)
                            gTATTCATAAACAGCCATTACCATCGATGCCGTTAt         >  1:6790987/1‑36 (MQ=255)
                            gTATTCATAAACAGCCATTACCATCGATGCCGTTAt         >  1:6871694/1‑36 (MQ=255)
                            gTATTCATAAACAGCCATTACCATCGATCACGTTAt         >  1:3973870/1‑36 (MQ=37)
                             tATTCATAAACAGCCATTACCATCGATGCCGTTATg        >  1:5666740/1‑36 (MQ=255)
                              aTTCATAAACAGCCATTACCATCGATGCCGTTATgg       >  1:5534709/1‑36 (MQ=255)
                              aTTCATAAACAGCCATTACCATCGATGCCGTTATgg       >  1:3400033/1‑36 (MQ=255)
                                tCATAAACAGCCATTACCATCGATGCCGTTATGGCa     >  1:3366534/1‑36 (MQ=255)
                                 cATAAACAGCCATTACCATCGATGCCGTTATGGCaa    >  1:3298248/1‑36 (MQ=255)
                                 cATAAACAGCCATTACCATCGATGCCGTTATGGCaa    >  1:7858294/1‑36 (MQ=255)
                                  aTAAACAGCCATTACCATCGATGCCGTTATGGCaaa   >  1:2998812/1‑36 (MQ=255)
                                  aTAAACAGCCATTACCATCGATGCCGTTATGGCaaa   >  1:7811991/1‑36 (MQ=255)
                                  aTAAACAGCCATTACCATCGATGCCGTTATGGCaaa   >  1:1681685/1‑36 (MQ=255)
                                  aTAAACAGCCATTACCATCGATGCCGTTATGGCaaa   >  1:654851/1‑36 (MQ=255)
                                   tAAACAGCCATTACCATCGATGCCGTTATGGCAAAg  >  1:1725495/1‑36 (MQ=255)
                                   tAAACAGCCATTACCATCGATGCCGTTATGGCAAAg  >  1:6569457/1‑36 (MQ=255)
                                   |                                   
ATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAG  >  REL606/1544317‑1544387

Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 37 ≤  ATCG