Predicted mutations
position mutation 2K 5K 10K 15K 20K annotation gene description
15,395 G→A 6.0% intergenic (+99/‑48) dnaJ → / → insL‑1 chaperone Hsp40, co‑chaperone with DnaK/IS186 hypothetical protein
16,972 IS150 (–) +3 bp 86.1%100% intergenic (‑14/‑514) mokC ← / → nhaA regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter
74,220 G→T 14.4% L22F (TTG→TTT yabI → conserved inner membrane protein
161,041 T→G 100% N302H (AAC→CAC)  pcnB ← poly(A) polymerase I
166,265 C→A 28.4% A437E (GCA→GAA)  hrpB → predicted ATP‑dependent helicase
380,188 A→C 40.7%92.6%100% F239L (TTT→TTG araJ ← predicted transporter
430,835 C→T 100% intergenic (‑48/‑108) insL‑2 ← / → lon putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La
475,288 +G 100%100%100% coding (18/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
547,700 Δ8,224 bp 100%100%100% IS1‑mediated [nmpC][ECB_00513] [nmpC], ybcR, ybcS, ybcT, ybcU, ECB_00510, nohB, ECB_00512, [ECB_00513]
649,391 T→A 100%100%100%100% I471F (ATC→TTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
position mutation 2K 5K 10K 15K 20K annotation gene description
668,787 A→C 100% Y65D (TAC→GAC)  gltI ← glutamate and aspartate transporter subunit
683,496 A→C 100%100%100%100% V65G (GTT→GGT)  nagC ← DNA‑binding transcriptional dual regulator, repressor of N‑acetylglucosamine
751,788 G→T 21.3% intergenic (+295/‑552) ybgG → / → cydA alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
851,606 G→A 18.4% S3F (TCT→TTT)  ybiU ← hypothetical protein
910,316 T→C 100% intergenic (+176/‑175) ybjN → / → potF predicted oxidoreductase/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
980,180 A→G 60.3% D365G (GAC→GGC)  rpsA → 30S ribosomal protein S1
980,293 3 bp x 2 7.6% duplication rpsA → 30S ribosomal protein S1
1,004,251 T→C 100% intergenic (‑177/+425) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
1,248,380 A→C 87.0%100% D295E (GAT→GAG dhaM ← fused predicted dihydroxyacetone‑specific PTS enzymes: HPr component/EI component
1,286,699 C→A 100% intergenic (+23/‑160) narI → / → ECB_01206 nitrate reductase 1, gamma (cytochrome b(NR)) subunit/hypothetical protein
position mutation 2K 5K 10K 15K 20K annotation gene description
1,329,516 C→T 100%100%100%100%100% H33Y (CAC→TAC)  topA → DNA topoisomerase I
1,370,728 G→T 17.0% W151L (TGG→TTG)  ycjN → predicted sugar transporter subunit: periplasmic‑binding component of ABC superfamily
1,733,288 8 bp x 2 17.7% duplication pykF → pyruvate kinase
1,733,579 7 bp x 2 49.5% duplication pykF → pyruvate kinase
1,733,647 IS150 (–) +3 bp 18.4%98.1%98.2%100%100% coding (683‑685/1413 nt) pykF → pyruvate kinase
1,764,698 IS1 (–) +9 bp 52.5% intergenic (‑318/‑7) ppsA ← / → ydiA phosphoenolpyruvate synthase/hypothetical protein
1,806,030 C→A 17.9% E324* (GAA→TAA)  astD ← succinylglutamic semialdehyde dehydrogenase
1,821,525 IS150 (–) +3 bp 96.3%100% coding (573‑575/1041 nt) ynjI ← predicted inner membrane protein
1,976,879 T→G 40.5%100% intergenic (‑57/‑76) yedW ← / → yedX predicted DNA‑binding response regulator in two‑component system with YedV/hypothetical protein
1,992,583 Δ1 bp 9.1% coding (942/1317 nt) shiA → shikimate transporter
position mutation 2K 5K 10K 15K 20K annotation gene description
2,082,685 G→A 16.2%100%100%92.5%100% A494V (GCT→GTT)  yegI ← hypothetical protein
2,329,291 A→T 20.6% A22A (GCA→GCT elaD → predicted enzyme
2,336,064 T→G 15.4% I168L (ATC→CTC)  nuoM ← NADH dehydrogenase subunit M
2,342,867 T→G 13.4% Q299P (CAG→CCG)  nuoG ← NADH dehydrogenase subunit G
2,499,315 G→A 36.6% intergenic (‑110/‑179) maeB ← / → talA malic enzyme/transaldolase A
2,732,014 C→A 34.6% R91L (CGC→CTC)  hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
3,015,771 IS150 (–) +4 bp 98.3% coding (1192‑1195/1677 nt) ECB_02816 → KpsD protein
3,045,069 G→T 46.7%100% T312N (ACC→AAC)  yghJ ← predicted inner membrane lipoprotein
3,155,168 G→A 93.0%100% G296S (GGC→AGC)  ebgR → DNA‑binding transcriptional repressor
3,248,957 A→T 100%100% D764E (GAT→GAA infB ← translation initiation factor IF‑2
position mutation 2K 5K 10K 15K 20K annotation gene description
3,288,092 G→A 100% R57C (CGT→TGT)  arcB ← hybrid sensory histidine kinase in two‑component regulatory system with ArcA
3,289,962 Δ16 bp 39.4%91.9%100% coding (96‑111/4554 nt) gltB → glutamate synthase, large subunit
3,293,142 G→T 21.1% L1092F (TTG→TTT gltB → glutamate synthase, large subunit
3,339,158 A→C 54.9%100% intergenic (+22/‑4) yhdG → / → fis tRNA‑dihydrouridine synthase B/DNA‑binding protein Fis
3,369,432 T→C 100% D50G (GAC→GGC)  rpsD ← 30S ribosomal protein S4
3,370,027 T→A 59.4%100%100% K117M (AAG→ATG)  rpsM ← 30S ribosomal protein S13
3,483,047 C→A 100%100%100% R455S (CGC→AGC)  malT → transcriptional regulator MalT
3,490,112 C→T 100% A105T (GCG→ACG)  glpE ← thiosulfate sulfurtransferase
3,750,741 C→A 19.1% L58F (TTG→TTT radC ← DNA repair protein RadC
3,762,741 A→T 100%100%100%100%100% K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
position mutation 2K 5K 10K 15K 20K annotation gene description
3,875,625 +T 100%100%100% intergenic (‑59/+294) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
3,893,550 +G 52.5% intergenic (+5/‑51) kup → / → insJ‑5 potassium transporter/IS150 hypothetical protein
3,894,997 Δ6,934 bp 100%100%100%100%100% IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
3,901,931 G→T ΔΔΔ 6.8%Δ T163T (ACC→ACA yieO ← predicted multidrug or homocysteine efflux system
4,100,655 C→T 16.6%100%100%100%100% M192I (ATG→ATA hslU ← ATP‑dependent protease ATP‑binding subunit
4,126,699 Δ1 bp 50.0%90.2%100% coding (335/879 nt) pflC → pyruvate formate lyase II activase
4,141,009 G→A 23.6% E28K (GAA→AAA)  yijC → DNA‑binding transcriptional repressor
4,192,978 G→C 100%? intergenic (+146/+147) rrfE → / ← yjaB 5S ribosomal RNA/predicted acetyltransferase
4,201,911 C→T 81.5%100% A217T (GCG→ACG)  iclR ← DNA‑binding transcriptional repressor
4,266,356 G→C 33.3% intergenic (‑52/‑341) acs ← / → nrfA acetyl‑coenzyme A synthetase/nitrite reductase, formate‑dependent, cytochrome
position mutation 2K 5K 10K 15K 20K annotation gene description
4,266,649 C→A 9.7% intergenic (‑345/‑48) acs ← / → nrfA acetyl‑coenzyme A synthetase/nitrite reductase, formate‑dependent, cytochrome
4,366,935 G→T 24.2% A197A (GCC→GCA yjeP ← predicted mechanosensitive channel
4,524,522 IS186 (+) +6 bp 25.9%68.8%93.5%98.5% coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)
4,560,632 T→C 30.9% Y131C (TAC→TGC)  hsdM ← DNA methylase M
4,561,446 C→G 9.6% L1097L (CTG→CTC hsdR ← endonuclease R
4,561,448 G→T 7.6% L1097M (CTG→ATG)  hsdR ← endonuclease R
4,616,396 G→A 9.9%100%100% G290S (GGC→AGC)  nadR → nicotinamide‑nucleotide adenylyltransferase