|
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
15,395 |
G→A |
| | | | 6.0% |
intergenic (+99/‑48) |
dnaJ → / → insL‑1 |
chaperone Hsp40, co‑chaperone with DnaK/IS186 hypothetical protein |
16,972 |
IS150 (–) +3 bp |
| | | 86.1% | 100% |
intergenic (‑14/‑514) |
mokC ← / → nhaA |
regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter |
74,220 |
G→T |
| | 14.4% | | |
L22F (TTG→TTT) |
yabI → |
conserved inner membrane protein |
161,041 |
T→G |
| | | | 100% |
N302H (AAC→CAC) |
pcnB ← |
poly(A) polymerase I |
166,265 |
C→A |
| | 28.4% | | |
A437E (GCA→GAA) |
hrpB → |
predicted ATP‑dependent helicase |
380,188 |
A→C |
| | 40.7% | 92.6% | 100% |
F239L (TTT→TTG) |
araJ ← |
predicted transporter |
430,835 |
C→T |
| | | | 100% |
intergenic (‑48/‑108) |
insL‑2 ← / → lon |
putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La |
475,288 |
+G |
| | 100% | 100% | 100% |
coding (18/1677 nt) |
ybaL ← |
predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
547,700 |
Δ8,224 bp |
| | 100% | 100% | 100% |
IS1‑mediated |
[nmpC]–[ECB_00513] |
[nmpC], ybcR, ybcS, ybcT, ybcU, ECB_00510, nohB, ECB_00512, [ECB_00513] |
649,391 |
T→A |
| 100% | 100% | 100% | 100% |
I471F (ATC→TTC) |
mrdA ← |
transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
668,787 |
A→C |
| | | | 100% |
Y65D (TAC→GAC) |
gltI ← |
glutamate and aspartate transporter subunit |
683,496 |
A→C |
| 100% | 100% | 100% | 100% |
V65G (GTT→GGT) |
nagC ← |
DNA‑binding transcriptional dual regulator, repressor of N‑acetylglucosamine |
751,788 |
G→T |
| 21.3% | | | |
intergenic (+295/‑552) |
ybgG → / → cydA |
alpha‑mannosidase/cytochrome d terminal oxidase, subunit I |
851,606 |
G→A |
| | | 18.4% | |
S3F (TCT→TTT) |
ybiU ← |
hypothetical protein |
910,316 |
T→C |
| | | | 100% |
intergenic (+176/‑175) |
ybjN → / → potF |
predicted oxidoreductase/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily |
980,180 |
A→G |
| | 60.3% | | |
D365G (GAC→GGC) |
rpsA → |
30S ribosomal protein S1 |
980,293 |
3 bp x 2 |
7.6% | | | | |
duplication |
rpsA → |
30S ribosomal protein S1 |
1,004,251 |
T→C |
| | | | 100% |
intergenic (‑177/+425) |
ompF ← / ← asnS |
outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
1,248,380 |
A→C |
| | | 87.0% | 100% |
D295E (GAT→GAG) |
dhaM ← |
fused predicted dihydroxyacetone‑specific PTS enzymes: HPr component/EI component |
1,286,699 |
C→A |
| | | | 100% |
intergenic (+23/‑160) |
narI → / → ECB_01206 |
nitrate reductase 1, gamma (cytochrome b(NR)) subunit/hypothetical protein |
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
1,329,516 |
C→T |
100% | 100% | 100% | 100% | 100% |
H33Y (CAC→TAC) |
topA → |
DNA topoisomerase I |
1,370,728 |
G→T |
| | | 17.0% | |
W151L (TGG→TTG) |
ycjN → |
predicted sugar transporter subunit: periplasmic‑binding component of ABC superfamily |
1,733,288 |
8 bp x 2 |
17.7% | | | | |
duplication |
pykF → |
pyruvate kinase |
1,733,579 |
7 bp x 2 |
49.5% | | | | |
duplication |
pykF → |
pyruvate kinase |
1,733,647 |
IS150 (–) +3 bp |
18.4% | 98.1% | 98.2% | 100% | 100% |
coding (683‑685/1413 nt) |
pykF → |
pyruvate kinase |
1,764,698 |
IS1 (–) +9 bp |
| | 52.5% | | |
intergenic (‑318/‑7) |
ppsA ← / → ydiA |
phosphoenolpyruvate synthase/hypothetical protein |
1,806,030 |
C→A |
17.9% | | | | |
E324* (GAA→TAA) |
astD ← |
succinylglutamic semialdehyde dehydrogenase |
1,821,525 |
IS150 (–) +3 bp |
| | | 96.3% | 100% |
coding (573‑575/1041 nt) |
ynjI ← |
predicted inner membrane protein |
1,976,879 |
T→G |
| | 40.5% | | 100% |
intergenic (‑57/‑76) |
yedW ← / → yedX |
predicted DNA‑binding response regulator in two‑component system with YedV/hypothetical protein |
1,992,583 |
Δ1 bp |
| | 9.1% | | |
coding (942/1317 nt) |
shiA → |
shikimate transporter |
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
2,082,685 |
G→A |
16.2% | 100% | 100% | 92.5% | 100% |
A494V (GCT→GTT) |
yegI ← |
hypothetical protein |
2,329,291 |
A→T |
| | 20.6% | | |
A22A (GCA→GCT) |
elaD → |
predicted enzyme |
2,336,064 |
T→G |
| | 15.4% | | |
I168L (ATC→CTC) |
nuoM ← |
NADH dehydrogenase subunit M |
2,342,867 |
T→G |
| | 13.4% | | |
Q299P (CAG→CCG) |
nuoG ← |
NADH dehydrogenase subunit G |
2,499,315 |
G→A |
| | 36.6% | | |
intergenic (‑110/‑179) |
maeB ← / → talA |
malic enzyme/transaldolase A |
2,732,014 |
C→A |
| | | | 34.6% |
R91L (CGC→CTC) |
hypF ← |
carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases |
3,015,771 |
IS150 (–) +4 bp |
| | | | 98.3% |
coding (1192‑1195/1677 nt) |
ECB_02816 → |
KpsD protein |
3,045,069 |
G→T |
| | 46.7% | | 100% |
T312N (ACC→AAC) |
yghJ ← |
predicted inner membrane lipoprotein |
3,155,168 |
G→A |
| | | 93.0% | 100% |
G296S (GGC→AGC) |
ebgR → |
DNA‑binding transcriptional repressor |
3,248,957 |
A→T |
| | 100% | | 100% |
D764E (GAT→GAA) |
infB ← |
translation initiation factor IF‑2 |
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
3,288,092 |
G→A |
| | | | 100% |
R57C (CGT→TGT) |
arcB ← |
hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
3,289,962 |
Δ16 bp |
| | 39.4% | 91.9% | 100% |
coding (96‑111/4554 nt) |
gltB → |
glutamate synthase, large subunit |
3,293,142 |
G→T |
21.1% | | | | |
L1092F (TTG→TTT) |
gltB → |
glutamate synthase, large subunit |
3,339,158 |
A→C |
| | 54.9% | | 100% |
intergenic (+22/‑4) |
yhdG → / → fis |
tRNA‑dihydrouridine synthase B/DNA‑binding protein Fis |
3,369,432 |
T→C |
| | | | 100% |
D50G (GAC→GGC) |
rpsD ← |
30S ribosomal protein S4 |
3,370,027 |
T→A |
| | 59.4% | 100% | 100% |
K117M (AAG→ATG) |
rpsM ← |
30S ribosomal protein S13 |
3,483,047 |
C→A |
| | 100% | 100% | 100% |
R455S (CGC→AGC) |
malT → |
transcriptional regulator MalT |
3,490,112 |
C→T |
| | | | 100% |
A105T (GCG→ACG) |
glpE ← |
thiosulfate sulfurtransferase |
3,750,741 |
C→A |
19.1% | | | | |
L58F (TTG→TTT) |
radC ← |
DNA repair protein RadC |
3,762,741 |
A→T |
100% | 100% | 100% | 100% | 100% |
K662I (AAA→ATA) |
spoT → |
bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
3,875,625 |
+T |
| 100% | 100% | | 100% |
intergenic (‑59/+294) |
glmU ← / ← atpC |
bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
3,893,550 |
+G |
| | 52.5% | | |
intergenic (+5/‑51) |
kup → / → insJ‑5 |
potassium transporter/IS150 hypothetical protein |
3,894,997 |
Δ6,934 bp |
100% | 100% | 100% | 100% | 100% |
IS150‑mediated |
rbsD–[yieO] |
rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
3,901,931 |
G→T |
Δ | Δ | Δ | 6.8% | Δ |
T163T (ACC→ACA) |
yieO ← |
predicted multidrug or homocysteine efflux system |
4,100,655 |
C→T |
16.6% | 100% | 100% | 100% | 100% |
M192I (ATG→ATA) |
hslU ← |
ATP‑dependent protease ATP‑binding subunit |
4,126,699 |
Δ1 bp |
| | 50.0% | 90.2% | 100% |
coding (335/879 nt) |
pflC → |
pyruvate formate lyase II activase |
4,141,009 |
G→A |
| | | 23.6% | |
E28K (GAA→AAA) |
yijC → |
DNA‑binding transcriptional repressor |
4,192,978 |
G→C |
| 100% | | | ? |
intergenic (+146/+147) |
rrfE → / ← yjaB |
5S ribosomal RNA/predicted acetyltransferase |
4,201,911 |
C→T |
| | | 81.5% | 100% |
A217T (GCG→ACG) |
iclR ← |
DNA‑binding transcriptional repressor |
4,266,356 |
G→C |
| | 33.3% | | |
intergenic (‑52/‑341) |
acs ← / → nrfA |
acetyl‑coenzyme A synthetase/nitrite reductase, formate‑dependent, cytochrome |
position |
mutation |
2K |
5K |
10K |
15K |
20K |
annotation |
gene |
description |
4,266,649 |
C→A |
| 9.7% | | | |
intergenic (‑345/‑48) |
acs ← / → nrfA |
acetyl‑coenzyme A synthetase/nitrite reductase, formate‑dependent, cytochrome |
4,366,935 |
G→T |
| | 24.2% | | |
A197A (GCC→GCA) |
yjeP ← |
predicted mechanosensitive channel |
4,524,522 |
IS186 (+) +6 bp |
25.9% | | 68.8% | 93.5% | 98.5% |
coding (494‑499/549 nt) |
fimA → |
major type 1 subunit fimbrin (pilin) |
4,560,632 |
T→C |
| | 30.9% | | |
Y131C (TAC→TGC) |
hsdM ← |
DNA methylase M |
4,561,446 |
C→G |
| | | 9.6% | |
L1097L (CTG→CTC) |
hsdR ← |
endonuclease R |
4,561,448 |
G→T |
| | | 7.6% | |
L1097M (CTG→ATG) |
hsdR ← |
endonuclease R |
4,616,396 |
G→A |
| | 9.9% | 100% | 100% |
G290S (GGC→AGC) |
nadR → |
nicotinamide‑nucleotide adenylyltransferase |