Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,437,3660C→T 6.8% 1.4 ‑3.8 0.071534yjfFpredicted sugar transporter subunit: membrane component of ABC superfamily
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (29/36):  total (35/36)
Fisher's exact test strand distribution p-value = 2.30e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

TGCTGATGTTGGCGGTGGTGGTTATCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATA  >  REL606/4437332‑4437401
                                  |                                   
tgctgATGTTGGCGGTGGTGGTTATCGGTATTTTcc                                    <  1:3689113/36‑1 (MQ=255)
tgctgATGTGGGCGGTGGTGGATATCGGTATTTTcc                                    <  1:2416508/36‑1 (MQ=37)
 gctgATGTTGGCGGTGGTGGTTATCGGTATTTTCCt                                   <  1:5162317/36‑1 (MQ=255)
  ctgATGTTGGCGGTGGTGGTTTTTGGTTTTTTTCtt                                  >  1:4460543/1‑35 (MQ=16)
  ctgATGTTGGCGGTGGTGGTTATCGGTATTTTCCTc                                  <  1:3375929/36‑1 (MQ=255)
  ctgATGTTGGCGGTGGGGGGTTTTGGGATTTTTCTc                                  >  1:2913159/1‑36 (MQ=9)
   tgATGTTGGCGGTGGTGGTTTTTGGTTTTTTTCTcg                                 >  1:5857405/1‑36 (MQ=16)
   tgATGTTGGCGGTGGTGGTTGTTGGTATTTTTCTcg                                 >  1:264713/1‑36 (MQ=21)
         tGGCGGTGGTGGTTATCGGTATTTTCCTCGCGCATc                           <  1:1626156/36‑1 (MQ=255)
          ggCGGTGGTGGTTATCGGTATTTTCCTCGCGCATCg                          <  1:1876498/36‑1 (MQ=255)
          ggCGGTGGTGGTTATCGGGATTTTGCTCCCGCagtg                          >  1:2098676/1‑33 (MQ=16)
          ggCGGTGGTGGGTATAGGGATTTTTCTCGCGCATCg                          >  1:1617773/1‑36 (MQ=16)
            cGGTGGTGGTTATCGGTATTTTCCTCGCGCATCGTa                        <  1:7136498/36‑1 (MQ=255)
            cGGTGGTGGTTATCGGTATTTTCCTCGCGCATCGTa                        >  1:3165095/1‑36 (MQ=255)
             ggtggtggtTATCGGTATTTTCCTCGCGCATCGTAc                       >  1:2010036/1‑36 (MQ=255)
             ggtggtggtTATCGGTATTTTCCTCGCGCATCGTAc                       <  1:1769321/36‑1 (MQ=255)
             ggtggtggtTATCGGTATTTTCCTCGCGCATCGTAc                       >  1:2163111/1‑36 (MQ=255)
             ggtggtggtTATCGGTATTTTCCTCGCGCATCGTAc                       >  1:3280033/1‑36 (MQ=255)
             ggtggtggtTATCGGTATTTTCCTCGCGCATCGTAc                       >  1:2880418/1‑36 (MQ=255)
              gtggtggtTATCGGTATTTTCCTCGCGCATCGTAcc                      <  1:5426650/36‑1 (MQ=255)
              gtggtggtTATCGGTATTTTCCTCGCGCATCGTAcc                      <  1:2451826/36‑1 (MQ=255)
               tggtggtTATCGGTATTTTCCTCGCGCATCGTAccc                     >  1:6905510/1‑36 (MQ=255)
               tggtggtTATCGGTATTTTCCTCGCGCATCGTAccc                     <  1:3373162/36‑1 (MQ=255)
               tggtggtTATCGGTATTTTCCTCGCGCATCGTAccc                     <  1:5599370/36‑1 (MQ=255)
               tggtggtTATCGGTATTTTCCTCGCGCATCGTAccc                     <  1:4026581/36‑1 (MQ=255)
               tggtggtTATCGGTATTTTCCTCGCGCATCGTAccc                     <  1:1781732/36‑1 (MQ=255)
                ggtggtTATCGGTATTTTCCTCGCGCATCGTACCCg                    >  1:453444/1‑36 (MQ=255)
                ggtggtTATCGGTATTTTCCTCGCGCATCGTACCCg                    <  1:2679291/36‑1 (MQ=255)
                ggtggtTATCGGTATTTTCCTCGCGCATCGTACCCg                    >  1:5337546/1‑36 (MQ=255)
                ggtggtTATCGGTATTTTCCTCGCGCATCGTACCCg                    >  1:4189352/1‑36 (MQ=255)
                 atggtTATCGGTATTTTCCTCGCGCATCGTACCCGt                   <  1:3113580/35‑1 (MQ=255)
                  tggtTATCGGTATTTTCCTCGCGCATCGTACCCGtt                  >  1:6023050/1‑36 (MQ=255)
                  tggtTATCGGTATTTTCCTCGCGCATCGTACCCGtt                  <  1:1746791/36‑1 (MQ=255)
                   ggtTATCGGTATTTTCCTCGCGCATCGTACCCGttt                 <  1:4657474/36‑1 (MQ=255)
                   ggtTATCGGTATTTTCCTCGCGCATCGTACCCGttt                 <  1:7104276/36‑1 (MQ=255)
                   ggtTATCGGTATTTTCCTCGCGCATCGTACCCGttt                 <  1:1055561/36‑1 (MQ=255)
                   ggtTATCGGTATTTTCCTCGCGCATCGTACCCGttt                 <  1:3187338/36‑1 (MQ=255)
                   ggtTATCGGTATTTTCCTCGCGCATCGTACCCGttt                 >  1:7881092/1‑36 (MQ=255)
                    gtTATCGGTATTTTCCTCGCGCATCGTACCCGtttt                >  1:6403715/1‑36 (MQ=255)
                    gtTATCGGTATTTTCCTCGCGCATCGTACCCGtttt                >  1:399611/1‑36 (MQ=255)
                     ttATCGGTATTTTCCTCGCGCATCGTACCCGTTTTg               >  1:3135104/1‑36 (MQ=255)
                     ttATCGGTATTTTCCTCGCGCATCGTACCCGTTTTg               >  1:2908983/1‑36 (MQ=255)
                      tATCGGTATTTTCCTCGCGCATCGTACCCGTTTTgg              <  1:7472565/36‑1 (MQ=255)
                       aTCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGt             <  1:7483068/36‑1 (MQ=255)
                       aTCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGt             >  1:6711781/1‑36 (MQ=255)
                       aTCGGTATTGTCCTCGCGCATCGTACCCGTTTTGGt             >  1:538116/1‑36 (MQ=255)
                        tCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTa            <  1:1182961/36‑1 (MQ=255)
                        tCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTa            <  1:4287065/36‑1 (MQ=255)
                        tCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTa            <  1:4622327/36‑1 (MQ=255)
                        tCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTa            >  1:1010059/1‑36 (MQ=255)
                        tCGGGATTTTCCTCGCGCATCGCACCCGTTTTGGTa            >  1:5616718/1‑36 (MQ=37)
                         cGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTaa           >  1:2980919/1‑36 (MQ=255)
                          ggTATTTTCCTCGCGCATCGTGCCCGTTTTGGTAAt          <  1:2062722/36‑1 (MQ=255)
                          ggTATTTTCCTCGCGCATCGTACCCGTTTTGGTNAt          >  1:4506558/1‑36 (MQ=255)
                          ggTATTTTCCTCGCGCATCGTACCCGTTTTGGTAAt          <  1:81880/36‑1 (MQ=255)
                          ggTATTTTCCTCGCGCATCGTACCCGTTTTGGTAAt          <  1:3194029/36‑1 (MQ=255)
                           gTATTTTCCTCGCGCATCGTACCCGTTTTGGTAATc         >  1:3757825/1‑36 (MQ=255)
                           gTATTTTCCTCGCGCATCGTACCCGTTTTGGTAATc         <  1:7433616/36‑1 (MQ=255)
                            tATTTTCCTCGCGCATCGTACCCGTTTTGGTAATCa        <  1:1711573/36‑1 (MQ=255)
                            tATTTTCCTCGCGCATCGTACCCGTTTTGGTAATCa        <  1:2264486/36‑1 (MQ=255)
                            tATTTTCCTCGCGCATCGTACCCGTTTTGGTAATCa        >  1:4121301/1‑36 (MQ=255)
                              ttttCCTCGCGCATCGTACCCGTTTTGGTAATCAgg      >  1:2280291/1‑36 (MQ=255)
                              ttttCCTCGCGCATCGTACCCGTTTTGGTAATCAgg      <  1:3471323/36‑1 (MQ=255)
                              ttttCCTCGCGCATCGTACCCGTTTTGGTAATCAgg      >  1:3302883/1‑36 (MQ=255)
                               tttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGt     >  1:6755308/1‑36 (MQ=255)
                               tttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGt     <  1:4318301/36‑1 (MQ=255)
                                ttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGta    <  1:3366013/36‑1 (MQ=255)
                                ttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGta    <  1:2801329/36‑1 (MQ=255)
                                ttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGta    <  1:3517389/36‑1 (MQ=255)
                                ttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGta    >  1:4446883/1‑36 (MQ=255)
                                ttCCTCGCGCATCGTACCCGTTTTGGTAATCAGGta    >  1:4927279/1‑36 (MQ=255)
                                 tCCTCGCGCATCGTACCCGTTTTGGTAATCAGGtat   >  1:2988789/1‑36 (MQ=255)
                                 tCCTCGCGCATCGTACCCGTTTTGGTAATCAGGtat   <  1:7184902/36‑1 (MQ=255)
                                  ccTCTCGCATCGTACCCGTTTTGGTAATCAGGtata  <  1:2040132/36‑1 (MQ=255)
                                  ccTCGCGCATCGTACCCGTTTTGGTAATCAGGtata  <  1:2435057/36‑1 (MQ=255)
                                  ccTCGCGCATCGTACCCGTTTTGGTAATCAGGtata  <  1:7678805/36‑1 (MQ=255)
                                  |                                   
TGCTGATGTTGGCGGTGGTGGTTATCGGTATTTTCCTCGCGCATCGTACCCGTTTTGGTAATCAGGTATA  >  REL606/4437332‑4437401

Base quality scores:  ATCG  < 3 ≤  ATCG  < 13 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 37 ≤  ATCG