Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 514,834 C→T 22.5% L261F (CTC→TTC)  glxK → glycerate kinase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL606514,8340C→T22.5% 2.8 ‑2.0 ‑0.025781glxKglycerate kinase II
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (11/8):  total (17/8)
Fisher's exact test strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.19e-01

GCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGTATTGAGATTGTG  >  REL606/514801‑514869
                                 |                                   
gcgggcggTATGGGTATTGCGGCGGCGGTATTtctc                                   <  1:116161/36‑1 (MQ=255)
gcgggcggTATGGGTATTGCGGCGGCGGGATTtttc                                   >  1:2490413/1‑36 (MQ=37)
 cgggcggTATGGGTATTGCGGCGGCGGTATTTCTCa                                  >  1:5440330/1‑36 (MQ=255)
 cgggcggTATGGGTATTGCGGCGGCGGTATTTCTCa                                  <  1:2829927/36‑1 (MQ=255)
  gggcggTATGGATATTGCGGCGGCGGGTTTTTTCaa                                 >  1:4307383/1‑36 (MQ=12)
     cggTATGGGTATTGCGGCGGCGGTATTTCTCAATGc                              <  1:772634/36‑1 (MQ=255)
        tATGGGTATTGCGGCGGCGGTATTTTTTAATTCGGa                           >  1:7459904/1‑36 (MQ=25)
        tATGGGTATTGCGGCGGCGGGATTTTTCAATGTggg                           >  1:5252231/1‑35 (MQ=21)
         aTGGGTATTGCGGCGGCGGTATTTTTCAATGCgggt                          >  1:6544100/1‑34 (MQ=37)
         aTGGGTATTGCGGCGGCGGTATTTCTCAATGCGGat                          <  1:3090982/36‑1 (MQ=255)
         aTGGGTATTGCGGCGGCGGGATTTTTCTATGCgggt                          >  1:4161688/1‑34 (MQ=21)
         aTGGGAATTGCGGCGGCGGTATTTCTCAATGCGGat                          <  1:7587590/36‑1 (MQ=255)
          tGGGTATTGCGGCGGCGGTATTTCTTAATGCGGgta                         >  1:4993565/1‑36 (MQ=37)
           gggTATTGCGGCGGCGGTGTTTCTTAATGCGGgtat                        >  1:3664310/1‑36 (MQ=21)
           gggTATTGCGGCGGCGGTATTTTTCAATGCGGatat                        >  1:3754540/1‑36 (MQ=255)
           gggTATTGCGGCGGCGGTATTTCTCAATGCGGatat                        <  1:6943693/36‑1 (MQ=255)
            ggTATTGCGGCGGCGGTATTTCTTAATGCGGATAtt                       >  1:4638445/1‑36 (MQ=255)
              tATTGCGGCGGCGGTATTTCTCAATGCGGATAttta                     >  1:4758934/1‑34 (MQ=255)
                ttGCGGCGGCGGTATTTCTCCATGCGGATATTAAAc                   >  1:129435/1‑36 (MQ=255)
                  gcggcggcgGTATTTCTCCATGCGGATATTTAACCg                 >  1:5472671/1‑36 (MQ=37)
                    ggcggcggTATTTCTCAATGCGGATATTAAACCggg               >  1:7124381/1‑36 (MQ=255)
                      cggcggTATTTCTCAATGCGGATATTAAACCGGGTa             <  1:1571998/36‑1 (MQ=255)
                       ggcggTATTTCTCAATGCGGATATTAAACCGGGTAt            <  1:3550392/36‑1 (MQ=255)
                         cggTATTTCTCAATGCGGATATTAAACCGGGTATTg          >  1:2055592/1‑36 (MQ=255)
                            tATTTCTCAATGCGGATATTAAACCGGGTATTgaga       >  1:2429961/1‑36 (MQ=255)
                             aTTTCTCAATGCGGATATTAAACCGGGTATTGAGAt      <  1:5736078/36‑1 (MQ=255)
                             aTTTCTCAATGCGGATATTAAACCGGGTATTGAGAt      >  1:1827521/1‑36 (MQ=255)
                                tctcAATGCGGATATTAAACCGGGTATTGAGATtgt   <  1:4375190/36‑1 (MQ=255)
                                tctcAATGCGGATATTAAACCGGGTATTGAGATtgt   <  1:3558538/36‑1 (MQ=255)
                                 ctcAATGCGGATATTAAGCCGGGTATTGAGATtgtg  <  1:1960908/36‑1 (MQ=255)
                                 ctcAATGCGGATATTAAACCGGGTATTGAGATtgtg  <  1:4521172/36‑1 (MQ=255)
                                 ctcAATGCGGATATTAAACCGGGTATTGAGATtgtg  <  1:2125849/36‑1 (MQ=255)
                                 |                                   
GCGGGCGGTATGGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGTATTGAGATTGTG  >  REL606/514801‑514869

Base quality scores:  ATCG  < 3 ≤  ATCG  < 6 ≤  ATCG  < 16 ≤  ATCG  < 27 ≤  ATCG  < 37 ≤  ATCG