| 1L | Component |
|---|---|
| 6 g | Sodium phosphate, Na2HPO4 (anhydrous) |
| 3 g | Potassium phosphate, KH2PO4 |
| 0.5 g | Sodium chloride, NaCl |
| 1 g | Ammonium chloride, NH4Cl |
| 16 g | Agar |
| 1 ml | Antifoam |
After media is autoclaved add the following sterile ingredients:
| 1L | Component |
|---|---|
| 1 ml | 1M Magnesium sulfate, MgSO4 |
| 1 ml | 0.1M Calcium chloride CaCl2 |
| 10 ml | 10% (w/v) glucose (or other carbon source) |