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TWiki's Lab web https://www.barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://www.barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif LabPersonnel https://www.barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by DevinLake) 2025-09-25T14:29:23Z DevinLake ContactInformation https://www.barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Distinguished Professor Department of Microbiology, Genetics, Immunology Department of Entomology... (last changed by DevinLake) 2025-09-25T00:29:54Z DevinLake PastLabPersonnel https://www.barricklab.org/twiki/bin/view/Lab/PastLabPersonnel Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake) 2025-09-24T16:08:36Z DevinLake PublicationList https://www.barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-09-22T01:08:03Z JeffreyBarrick WebHome https://www.barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-22T00:30:34Z JeffreyBarrick PublicationListAND238=68ANDbjVN!=bjVN https://www.barricklab.org/twiki/bin/view/Lab/PublicationListAND238=68ANDbjVN!=bjVN Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest) 2025-09-16T09:49:25Z guest PublicationListAND238=68ANDelB7=elB7 https://www.barricklab.org/twiki/bin/view/Lab/PublicationListAND238=68ANDelB7=elB7 Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest) 2025-09-16T09:49:21Z guest PublicationListAND238=68ANDoJht=oJht https://www.barricklab.org/twiki/bin/view/Lab/PublicationListAND238=68ANDoJht=oJht Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest) 2025-09-16T09:49:18Z guest WebHomeULAZujysvtYf https://www.barricklab.org/twiki/bin/view/Lab/WebHomeULAZujysvtYf .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by TWikiGuest) 2025-09-13T02:11:09Z guest WebHome__),,)__ https://www.barricklab.org/twiki/bin/view/Lab/WebHome__),,)__ .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by TWikiGuest) 2025-09-13T02:11:06Z guest ProtocolList https://www.barricklab.org/twiki/bin/view/Lab/ProtocolList Barrick Lab :: Laboratory Protocols Updating Protocols How to Create a Protocol Best practices for designing a protocol and for putting it on the lab Wiki.... (last changed by AlexaMorton) 2025-05-20T15:32:29Z AlexaMorton ProtocolsAcinetobacterGoldenTransformation https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterGoldenTransformation Acinetobacter baylyi ADP1 Golden Transformation Overview The following protocol is to be used as a substitute for overlap extension PCR for constructing double stranded... (last changed by MeghnaVergis) 2025-03-19T19:51:42Z MeghnaVergis CommandLineBox https://www.barricklab.org/twiki/bin/view/Lab/CommandLineBox Copying files to/from UT Box at the command line If you use SSO (through UT Box), you need to first up an external password. You will need LFTP installed on your system... (last changed by JeffreyBarrick) 2024-05-18T01:40:29Z JeffreyBarrick ProcedureDNASequencing https://www.barricklab.org/twiki/bin/view/Lab/ProcedureDNASequencing Sanger DNA Sequencing Go to the UT DNA Sequencing Facility website. This page explains the pricing for various orders and the methods by which samples should be prepared... (last changed by ZuberiAshraf) 2023-02-27T18:32:51Z ZuberiAshraf ProtocolsOrderingPrimers https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsOrderingPrimers Ordering Primers 1 Go to ICMB`s IDT webpage ICMB`s IDT page 1 Click Set up new Account tab on bottom left. 1 Fill out all of your information, including... (last changed by JeffreyBarrick) 2022-09-26T21:55:23Z JeffreyBarrick SupplementLongTermMutationRates https://www.barricklab.org/twiki/bin/view/Lab/SupplementLongTermMutationRates Supplementary Scripts and Data Files Mutation rate inferred from synonymous substitutions in a long term evolution experiment with Escherichia coli Wielgoss, et... (last changed by JeffreyBarrick) 2022-04-22T14:30:55Z JeffreyBarrick
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TWiki's Lab web https://www.barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://www.barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif LabPersonnel https://www.barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by DevinLake) 2025-09-25T14:29:23Z DevinLake ContactInformation https://www.barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Distinguished Professor Department of Microbiology, Genetics, Immunology Department of Entomology... (last changed by DevinLake) 2025-09-25T00:29:54Z DevinLake PastLabPersonnel https://www.barricklab.org/twiki/bin/view/Lab/PastLabPersonnel Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake) 2025-09-24T16:08:36Z DevinLake PublicationList https://www.barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-09-22T01:08:03Z JeffreyBarrick WebHome https://www.barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-22T00:30:34Z JeffreyBarrick PublicationListAND238=68ANDbjVN!=bjVN https://www.barricklab.org/twiki/bin/view/Lab/PublicationListAND238=68ANDbjVN!=bjVN Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest) 2025-09-16T09:49:25Z guest PublicationListAND238=68ANDelB7=elB7 https://www.barricklab.org/twiki/bin/view/Lab/PublicationListAND238=68ANDelB7=elB7 Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest) 2025-09-16T09:49:21Z guest PublicationListAND238=68ANDoJht=oJht https://www.barricklab.org/twiki/bin/view/Lab/PublicationListAND238=68ANDoJht=oJht Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest) 2025-09-16T09:49:18Z guest WebHomeULAZujysvtYf https://www.barricklab.org/twiki/bin/view/Lab/WebHomeULAZujysvtYf .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by TWikiGuest) 2025-09-13T02:11:09Z guest WebHome__),,)__ https://www.barricklab.org/twiki/bin/view/Lab/WebHome__),,)__ .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by TWikiGuest) 2025-09-13T02:11:06Z guest ProtocolsAntibioticStockSolutions https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsAntibioticStockSolutions Media amendments Preparation: Generally, prepare 30 50 mL of solution in a 50 mL conical tube. Then, filter sterilize solutions by pushing them through a 50 mL syringe... (last changed by AlexaMorton) 2025-06-11T19:01:08Z AlexaMorton ListofBioinformatics https://www.barricklab.org/twiki/bin/view/Lab/ListofBioinformatics Bioinformatics Master List This page is a master list of bioinformatics software frequently used in the lab, and their purposes. It also includes additional notes... (last changed by IraZibbu) 2025-04-17T21:59:52Z IraZibbu MicroplateReaderQuickStartGuide https://www.barricklab.org/twiki/bin/view/Lab/MicroplateReaderQuickStartGuide Microplate Reader Quick Start Best Practices 1 Follow typical best practice for experimental design. Utilize technical replicates from the same biological sample... (last changed by JeffreyBarrick) 2024-04-17T18:27:05Z JeffreyBarrick ProtocolsGoldenGateTroubleshooting https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsGoldenGateTroubleshooting Back to Golden Gate Protocols Troubleshooting Golden Gate Assemblies Tips Screen Inserts for internal BsaI/BsmBI sites! Reactions with single off target sites... (last changed by JeffreyBarrick) 2023-06-14T17:43:25Z JeffreyBarrick ProtocolsBTKAssembleMultipleTUPlasmid https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleMultipleTUPlasmid Back to Golden Gate Protocols Second Stage Assembly Second Stage assemblies are used to build plasmids out of multiple transcriptional units (Protocol found here... (last changed by KateElston) 2021-11-03T20:58:18Z KateElston ProtocolsPlasmidCopyNumber https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsPlasmidCopyNumber Plasmid copy number determination Plasmid copy number is known to vary depending on origin of replication and culture conditions refs . Typically, plasmids are referred... (last changed by MattMcGuffie) 2021-03-30T16:15:36Z MattMcGuffie
 

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