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Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Distinguished Professor Department of Microbiology, Genetics, Immunology Department of Entomology... (last changed by DevinLake)2025-09-25T00:29:54ZDevinLakeLabPersonnel
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Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake)2025-09-24T16:08:36ZDevinLakePublicationList
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Generating Overhangs If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB`s GETSET tool can be useful for generating a set of high fidelity... (last changed by AlexaMorton)2025-06-17T14:27:14ZAlexaMortonProceduresLongTermCompetitions
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Co culture Competition Assays The instructions below include the basic protocol. Be sure to check whether there are variations needed for your specific samples! For... (last changed by JeffreyBarrick)2025-06-05T11:20:32ZJeffreyBarrickProtocolsRecipesLysogenyBroth
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LB: Lysogeny Broth / Luria Bertani Medium (Miller) Rich media used for routine culture of E. coli and other bacteria at high cell densities. Add dH2O to desired... (last changed by SophiaLin)2024-09-05T22:00:19ZSophiaLinProtocolsLabeling
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Labeling Glassware and Other Containers All bottles, flasks, tubes, graduated cylinders, and beakers that contain liquid or any substance must be labeled with a description... (last changed by SophiaLin)2024-07-09T21:53:26ZSophiaLinProcedureDNASequencing
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Sanger DNA Sequencing Go to the UT DNA Sequencing Facility website. This page explains the pricing for various orders and the methods by which samples should be prepared... (last changed by ZuberiAshraf)2023-02-27T18:32:51ZZuberiAshrafProtocolsPlasmidCopyNumber
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Plasmid copy number determination Plasmid copy number is known to vary depending on origin of replication and culture conditions refs . Typically, plasmids are referred... (last changed by MattMcGuffie)2021-03-30T16:15:36ZMattMcGuffie
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Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake)2025-09-24T16:08:36ZDevinLakePublicationList
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TA: Tetrazolium Sugar (TA, TM, TL, ...) Combine in a 1 L flask: 500 mL Component 5 g Tryptone 500 mg Yeast Extract 2.5 g NaCl 8 g... (last changed by AlexaMorton)2025-01-09T20:04:48ZAlexaMortonProtocolsComputerSetup
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Learn Biocomputing General Resources / Courses Software Carpentry https://software carpentry.org/lessons/ Code Academy https://www.codecademy.com/ edX Computer... (last changed by JeffreyBarrick)2024-07-09T21:00:49ZJeffreyBarrick
> >
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Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake)2025-09-24T16:08:36ZDevinLakePublicationList
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Media Recipes When making media, make sure the autoclave bin you`re using contains water (DI or tap) at least half an inch deep to help prevent excess water loss... (last changed by AlexaMorton)2025-06-12T14:23:46ZAlexaMortonProtocolsTroubleshooting
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TA: Tetrazolium Sugar (TA, TM, TL, ...) Combine in a 1 L flask: 500 mL Component 5 g Tryptone 500 mg Yeast Extract 2.5 g NaCl 8 g... (last changed by AlexaMorton)2025-01-09T20:04:48ZAlexaMortonProtocolsComputerSetup
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Computer Setup for Bioinformatics and Computational Biology So, you want to harness the immense power of bioinformatics and computational biology for your science... (last changed by JeffreyBarrick)2024-07-09T21:10:27ZJeffreyBarrickProtocolsLearnBiocomputing
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Learn Biocomputing General Resources / Courses Software Carpentry https://software carpentry.org/lessons/ Code Academy https://www.codecademy.com/ edX Computer... (last changed by JeffreyBarrick)2024-07-09T21:00:49ZJeffreyBarrick
TWiki's Lab web
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The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World.en-usCopyright 2025 by contributing authorsTWiki Administrator [admin@barricklab.org]The contributing authors of TWikiTWikiHome - this site is powered by TWiki(R).Lab
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Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake)2025-09-24T16:08:36ZDevinLakePublicationList
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SOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by AlexaMorton)2025-07-03T16:59:00ZAlexaMortonProcedureGeneReplacements
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Allelic exchange using pKOV or pKOV style vectors Before beginning part 1: design primers 1 Insert should be ~1kb with approximately 500bp on either side of mutation... (last changed by TylerDeJong)2025-03-01T23:17:14ZTylerDeJongProtocolsRecipesMinimalGlucose
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MG: Minimal Glucose agar, aka DM: Davis Mingioli agar Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar... (last changed by AlexaMorton)2025-01-09T20:14:37ZAlexaMortonWebLeftBar
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Back to Golden Gate Protocols Old Golden Gate Assembly Protocols This page contains a collection of old protocols for Golden Gate Assembly. All these protocols should... (last changed by TylerDeJong)2024-04-09T20:54:38ZTylerDeJongProtocolsGrowthRates
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Measuring Microbial Growth Rates in a Plate Reader The following protocol can be used to determine the growth rate of a bacterial culture using a plate reader by measuring... (last changed by JeffreyBarrick)2024-01-04T19:58:43ZJeffreyBarrick
> >
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Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake)2025-09-24T16:08:36ZDevinLakePublicationList
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Autoclave Sterilization Overview Autoclaves heat their contents to 121... (last changed by AlexaMorton)2025-06-13T13:30:39ZAlexaMortonProtocolsConjugation
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General Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol... (last changed by KadenaCope)2024-09-25T18:17:22ZKadenaCopeProtocolsStyleGuide
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Barrick Lab Style Guide Writing a Scientific Paper Steps to Writing a Research Article (Original: Beth A. Fischer and Michael J. Zigmond, Survival Skills and... (last changed by JeffreyBarrick)2024-04-27T13:32:06ZJeffreyBarrickMicroplateReaderQuickStartGuide
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Microplate Reader Quick Start Best Practices 1 Follow typical best practice for experimental design. Utilize technical replicates from the same biological sample... (last changed by JeffreyBarrick)2024-04-17T18:27:05ZJeffreyBarrickQPCR
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Comparative Quantitative PCR (qPCR) Quantitative PCR (qPCR) is a tool routinely used for the quantitation of target nucleic acid sequences. If it is your first time... (last changed by DanielDeatherage)2023-01-11T18:35:10ZDanielDeatherage
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Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Distinguished Professor Department of Microbiology, Genetics, Immunology Department of Entomology... (last changed by DevinLake)2025-09-25T00:29:54ZDevinLakeLabPersonnel
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Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by DevinLake)2025-09-24T21:25:34ZDevinLakePastLabPersonnel
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Past Barrick Lab Members Anna Grove Krisha Chaudhari Korin Jones Dr. Patrick Lariviere Dr. Dennis Mishler Alexa Morton Cameron Roots Jeffrey Chuong Kadena Cope Sophia... (last changed by DevinLake)2025-09-24T16:08:36ZDevinLakePublicationList
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Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)2025-09-22T01:08:03ZJeffreyBarrickWebHome
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Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest)2025-09-16T09:49:25ZguestPublicationListAND238=68ANDelB7=elB7
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Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest)2025-09-16T09:49:21ZguestPublicationListAND238=68ANDoJht=oJht
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Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by TWikiGuest)2025-09-16T09:49:18ZguestWebHomeULAZujysvtYf
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.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by TWikiGuest)2025-09-13T02:11:06ZguestProtocolsAntibioticStockSolutions
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Media amendments Preparation: Generally, prepare 30 50 mL of solution in a 50 mL conical tube. Then, filter sterilize solutions by pushing them through a 50 mL syringe... (last changed by AlexaMorton)2025-06-11T19:01:08ZAlexaMortonProtocolsAcinetobacterOverlapPCRTransformation
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Acinetobacter baylyi ADP1 Genome Manipulations by Overlap Extension PCR Overlap PCR Construct Generation The following is a standard procedure designing and constructing... (last changed by CameronRoots)2025-03-20T21:37:57ZCameronRootsProtocolsResuspendingPrimers
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(last changed by CameronRoots)2025-03-20T21:22:53ZCameronRootsBioPythonOnboard
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(last changed by JeffreyBarrick)2023-09-20T15:41:36ZJeffreyBarrickFreezerFailure
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Sensaphone Protocol The sensaphone is the alarm monitor connected to both of the 80C freezers in MBB. Below is an overview of the current settings and the expected... (last changed by DanielDeatherage)2023-01-11T20:06:04ZDanielDeatherageExperimentalqPCR
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C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Conditions If you want to be extra safe you should also include a negative control (usually... (last changed by KateElston)2022-07-19T23:00:43ZKateElston