TWiki's Lab webThe Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise.https://www.barricklab.org/twiki/bin/view/LabCopyright 2025 by contributing authors2025-09-05T18:23:35ZWebTopMenuhttps://www.barricklab.org/twiki/bin/view/Lab/WebTopMenu2025-09-05T18:23:35ZTop Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick)JeffreyBarrickWebStatisticshttps://www.barricklab.org/twiki/bin/view/Lab/WebStatistics2025-09-02T07:05:11ZStatistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)TWikiGuestWebHomehttps://www.barricklab.org/twiki/bin/view/Lab/WebHome2025-09-01T17:23:56Z.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)JeffreyBarrickFrontPageImageshttps://www.barricklab.org/twiki/bin/view/Lab/FrontPageImages2025-09-01T11:41:29ZAttach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick)JeffreyBarrickPublicationListhttps://www.barricklab.org/twiki/bin/view/Lab/PublicationList2025-08-31T14:27:55ZBarrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)JeffreyBarrickTopMenuSkinTopBarhttps://www.barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar2025-08-29T11:39:17ZTop Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick)JeffreyBarrickLabPersonnelhttps://www.barricklab.org/twiki/bin/view/Lab/LabPersonnel2025-08-29T11:15:46ZBarrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)JeffreyBarrickWebTopBarhttps://www.barricklab.org/twiki/bin/view/Lab/WebTopBar2025-08-29T01:24:36Z (last changed by JeffreyBarrick)JeffreyBarrickContactInformationhttps://www.barricklab.org/twiki/bin/view/Lab/ContactInformation2025-08-15T16:29:20ZBarrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick)JeffreyBarrickProceduresLongTermCompetitionshttps://www.barricklab.org/twiki/bin/view/Lab/ProceduresLongTermCompetitions2025-06-05T11:20:32ZCo culture Competition Assays The instructions below include the basic protocol. Be sure to check whether there are variations needed for your specific samples! For... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsTaqhttps://www.barricklab.org/twiki/bin/view/Lab/ProtocolsTaq2025-03-20T21:27:28ZWhich polymerase is right for me? Types of polymerase NEB polymerases may be purchased from the NEB freezer, which is located in the Biomedical Research Supply Core... (last changed by MeghnaVergis)MeghnaVergisProtocolsWorkingWithSerratiaSymbioticahttps://www.barricklab.org/twiki/bin/view/Lab/ProtocolsWorkingWithSerratiaSymbiotica2025-03-20T21:24:12ZWorking with Serratia symbiotica Serratia symbiotica is a secondary endosymbiont of the black bean aphid ( Aphis fabae ) gut microbiota that can be cultivated... (last changed by AnthonyVandieren)AnthonyVandierenReferenceListhttps://www.barricklab.org/twiki/bin/view/Lab/ReferenceList2025-03-20T20:47:39ZBarrick Lab :: Reference Information Getting Started in Lab Getting Started with Lab Techniques Synthetic Biology 101 Background on what synthetic biology... (last changed by IraZibbu)IraZibbuPhenolChloroformExtractionhttps://www.barricklab.org/twiki/bin/view/Lab/PhenolChloroformExtraction2022-07-20T16:42:59ZPhenol/chloroform extraction Extracting genomic bacterial DNA from pellet with phenol/chloroform with a combined EtOH precipitation step for salt/SDS/small nucleic... (last changed by MattMcGuffie)MattMcGuffieProtocolsAcinetobacterBaylyiADP1https://www.barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterBaylyiADP12022-03-22T18:35:13ZAcinetobacter baylyi ADP1 Overview ADP1 Resources Genome Sequences type strain (GenBank Format) In general, use this genome as a reference when referring... (last changed by IsaacGifford)IsaacGiffordProtocolsJournalArticleAccesshttps://www.barricklab.org/twiki/bin/view/Lab/ProtocolsJournalArticleAccess2021-02-13T15:11:59ZAccessing Journal Articles from Off Campus PubMed PubMed is often the best practice for biology researchers to find and discover publications related to biological... (last changed by JeffreyBarrick)JeffreyBarrick
TWiki's Lab webThe Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise.https://www.barricklab.org/twiki/bin/view/LabCopyright 2025 by contributing authors2025-09-05T18:23:35ZWebTopMenuhttps://www.barricklab.org/twiki/bin/view/Lab/WebTopMenu2025-09-05T18:23:35ZTop Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick)JeffreyBarrickWebStatisticshttps://www.barricklab.org/twiki/bin/view/Lab/WebStatistics2025-09-02T07:05:11ZStatistics for Lab Web Month: Topic views: Topic saves: File uploads: Most popular topic views: Top contributors for topic save and... (last changed by TWikiGuest)TWikiGuestWebHomehttps://www.barricklab.org/twiki/bin/view/Lab/WebHome2025-09-01T17:23:56Z.within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick)JeffreyBarrickFrontPageImageshttps://www.barricklab.org/twiki/bin/view/Lab/FrontPageImages2025-09-01T11:41:29ZAttach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick)JeffreyBarrickPublicationListhttps://www.barricklab.org/twiki/bin/view/Lab/PublicationList2025-08-31T14:27:55ZBarrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick)JeffreyBarrickTopMenuSkinTopBarhttps://www.barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar2025-08-29T11:39:17ZTop Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick)JeffreyBarrickLabPersonnelhttps://www.barricklab.org/twiki/bin/view/Lab/LabPersonnel2025-08-29T11:15:46ZBarrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick)JeffreyBarrickWebTopBarhttps://www.barricklab.org/twiki/bin/view/Lab/WebTopBar2025-08-29T01:24:36Z (last changed by JeffreyBarrick)JeffreyBarrickContactInformationhttps://www.barricklab.org/twiki/bin/view/Lab/ContactInformation2025-08-15T16:29:20ZBarrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick)JeffreyBarrickBroadHostRangeToolkithttps://www.barricklab.org/twiki/bin/view/Lab/BroadHostRangeToolkit2024-12-13T17:34:59ZBee Microbiome Toolkit The Bee Microbiome Toolkit (BTK) is a Golden Gate compatible toolkit of genetic parts designed for working with newly isolated, non model bacteria... (last changed by CameronRoots)CameronRootsWebLeftBarhttps://www.barricklab.org/twiki/bin/view/Lab/WebLeftBar2024-07-09T20:47:11ZBarrick Lab Contact Research Publications Team Protocols Reference Software UT Austin Mol Biosciences... (last changed by JeffreyBarrick)JeffreyBarrickProceduresGeneGorgingEvolvedAlleleshttps://www.barricklab.org/twiki/bin/view/Lab/ProceduresGeneGorgingEvolvedAlleles2024-01-12T17:09:54ZGene Gorging Evolved Alleles This procedure can be used to directly create unmarked mutations in the E. coli genome. Transform Gene Gorging and Donor Plasmid... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsGrowthRateshttps://www.barricklab.org/twiki/bin/view/Lab/ProtocolsGrowthRates2024-01-04T19:58:43ZMeasuring Microbial Growth Rates in a Plate Reader The following protocol can be used to determine the growth rate of a bacterial culture using a plate reader by measuring... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsRecipesABMShttps://www.barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesABMS2019-06-23T12:09:45ZABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following pre sterilized... (last changed by JeffreyBarrick)JeffreyBarrickIntroductionToExperimentalDesignhttps://www.barricklab.org/twiki/bin/view/Lab/IntroductionToExperimentalDesign2018-10-26T01:12:31ZIntroduction to Experimental Design Motivation Whether for just a summer or for the duration of an entire Ph.D project, working on a scientific problem is a process... (last changed by JeffreyBarrick)JeffreyBarrickProtocolsFluorescentProteinEvolutionaryStabilityhttps://www.barricklab.org/twiki/bin/view/Lab/ProtocolsFluorescentProteinEvolutionaryStability2018-03-22T19:37:19ZEvolutionary Stability of Fluorescent Protein or Chromoprotein Expression This protocol is a work in progress This procedure is to monitor the decay of a genetic... (last changed by JeffreyBarrick)JeffreyBarrick