Difference: ProtocolsTaq (2 vs. 3)
Revision 32011-10-12 - JeffreyBarrick
| |||||||||||
| Changed: | |||||||||||
| < < | How to select the right polymerase | ||||||||||
| > > | Which polymerase is right for me? | ||||||||||
| Changed: | |||||||||||
| < < | Types of polymeraseOur lab carries 2 main types of polymerase: | ||||||||||
| > > | Types of polymeraseOur lab stocks two main types of polymerase: | ||||||||||
| |||||||||||
| Changed: | |||||||||||
| < < |
| ||||||||||
| > > |
| ||||||||||
| Changed: | |||||||||||
| < < | Applications | ||||||||||
| > > | Applications | ||||||||||
|
Phusion High Fidelity High-fidelity PCR The error rate of Phusion DNA Polymerase is 4.4 x 10^-7; therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy. Cloning Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase possesses 5' - 3' polymerase activity and 3'-5' exonuclease activity and will generate blunt ended products. Long amplicons Extension and overall cycling times can be significantly reduced when using Phusion DNA Polymerase. Due to the high processivity, Phusion DNA Polymerase can amplify long templates in a fraction of the time other polymerases need.
| |||||||||||
| Added: | |||||||||||
| > > |
| ||||||||||
View topic | History: r8 < r7 < r6 < r5 | More topic actions...
