Difference: ProtocolsTaq (1 vs. 2)

Revision 22011-10-06 - CraigBarnhart

META TOPICPARENT	name="ProtocolList"

How to select the right polymerase

Types of polymerase

Our lab carries 2 main types of polymerase:

Type	Vender	Cost per unit	Product Information	Image
Taq DNA polymerase	Syzygy Biotech	0.18	link	Standard Taq

Changed:

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<

Phusion High Fidelity DNA poly

NEB

0.86

Info about phusion

Phusion HF

>
>

Phusion High Fidelity DNA poly

NEB

0.86

link

Phusion HF

Applications

Phusion High Fidelity

High-fidelity PCR

Changed:

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<

The error rate of Phusion DNA Polymerase is 4.4 x 10^-7, which is industry-leading fidelity. Therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.

>
>

The error rate of Phusion DNA Polymerase is 4.4 x 10^-7; therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.

Cloning

Changed:

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<

Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase produces blunt ends to the amplified fragment.

>
>

Long amplicons
Extension and overall cycling times can be significantly reduced when using Phusion DNA Polymerase. Due to the high processivity, Phusion DNA Polymerase can amplify long templates in a fraction of the time other polymerases need.

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Revision 12011-10-06 - CraigBarnhart

META TOPICPARENT	name="ProtocolList"

How to select the right polymerase

Types of polymerase

Our lab carries 2 main types of polymerase:

Type	Vender	Cost per unit	Product Information	Image
Taq DNA polymerase	Syzygy Biotech	0.18	link	Standard Taq
Phusion High Fidelity DNA poly	NEB	0.86	Info about phusion	Phusion HF

Applications

Phusion High Fidelity

High-fidelity PCR
The error rate of Phusion DNA Polymerase is 4.4 x 10^-7, which is industry-leading fidelity. Therefore, Phusion DNA Polymerase is suitable for all PCR applications requiring great accuracy.

Cloning
Phusion DNA Polymerase amplifies templates with an accuracy and speed previously unattainable with a single enzyme. This makes it a superior choice for cloning. Phusion DNA Polymerase produces blunt ends to the amplified fragment.

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META FILEATTACHMENT	attachment="Taq.JPG" attr="h" comment="Syzygy taq" date="1317911058" name="Taq.JPG" path="Taq.JPG" size="15346" stream="Taq.JPG" tmpFilename="/usr/tmp/CGItemp24032" user="CraigBarnhart" version="1"
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