Difference: ProtocolsRecipesTetrazoliumSugar (7 vs. 8)

Revision 82025-01-09 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Changed:
<
<		Combine in a 2 L flask:
1.5L Component
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.
>
>		Combine in a 1 L flask:
500 mL Component
5 g Tryptone
500 mg Yeast Extract
2.5 g NaCl
8 g Agar
500 µL 5% Antifoam
Add dH2O to 433.33 mL.
 
Changed:
<
<		Combine in a 500 ml flask:
1.5L Component
15 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.
>
>		Combine in a 125 ml flask:
500 mL Component
5 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 66.67 mL.
 
Changed:
<
<		Autoclave sugar and media separately. Total combined volume is 1.5 liters.
>
>		Cover each flask with foil, and autoclave sugar and media separately. Total combined volume will be 500 mL.
 
Changed:
<
<		Combine and add:
1.5L Component
1.5 ml 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
>
>		Combine the autoclaved sugar and media solutions and add:
500 mL Component
500 µL 5% (w/v) Triphenyl Tetrazolium chloride (TTC), filter sterilized, stored at 4°C
  Source: Lenski Lab Protocol
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