Difference: ProtocolsRecipesSaline (1 vs. 2)

Revision 22011-05-19 - JeffreyBarrick

Sterile Saline

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Purpose: Used make dilutions of viable cells for plating or transfer to new media.

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L	5L	component
8.5 g	42.5 g	NaCl

Add H₂O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.

Revision 12011-03-08 - JeffreyBarrick

Sterile Saline

This saline concentration of 0.85% w/v (145 mM) is suitable for diluting E. coli before transferring to new media or plating on agar.

1L	5L	component
8.5 g	42.5 g	NaCl

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