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| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
SOB/SOC: Super Optimal Broth
For SOB:
| 1L |
Final [ ] |
Component |
| 5 g |
0.5% |
yeast extract |
| 20 g |
2.0% |
tryptone |
| 0.5 g |
10 mM |
NaCl |
| 0.186 g |
2 mM |
KCl |
| 2.4 g |
20 mM |
MgSO4 (anhydrous) |
Add 800 ml of dH2O and dissolve components. Adjust to pH 7.5 prior to use with 1 M NaOH (approximately 25 ml). Add dH2O to a final volume 1 L. Autoclave
For SOC (=SOB+glucose):
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< | Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% glucose. |
>
> | Make SOB, except add dH2O to a final volume of 960 ml instead of 1 L. Autoclave. When solution has cooled to 50°C (cool enough to touch with bare hands for a few seconds), add 40 ml of sterile 10% (w/v) glucose. (That's a final concentration of 0.4% glucose.) |
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Note: Some recipes for SOB use 10 mM MgSO4 and 10 mM MgCl. |
