Difference: ProtocolsRecipesS2 (4 vs. 5)

Revision 52013-06-27 - AurkoDasgupta

 

S2

Used for Acinetobacter

1L 5L Component
1.5g 7.5 g KH2PO4
25.5g 127.5 g Na2HPO4 7H2O
Deleted:
<
<
0.1g 0.5 g MgSO4
 
2g 10 g NH4Cl
Deleted:
<
<
0.01g 0.05 g CaCl2
0.0005g 0.0025 g FeSO4 7H2O
 
Added:
>
>		After autoclaving, add the following sterile ingredients.
 
Changed:
<
<		To each 1L, add 5ml of 85-90% lactic acid. Bring final pH to 6.65.
>
>
1L Component
Added:
>
>
1ml 10% MgSO4
0.09ul 1M CaCl2
0.5ml 0.1% FeSO4 7H2O
5 ml 90% Lactic Acid
 
Changed:
<
<		Calcium Chloride may be better added as 1ml of 1% solution, or 90ul of 1M solution.
>
>		The functional constraints are that Calcium Chloride cannot be autoclaved in the presence of sulfates, or it will precipitate as calcium sulfate. Ferrous sulfate when autoclaved will decompose to ferric sulfate, ferric oxide and sulfur dioxide. This means that either calcium chloride OR magnesium sulfate may be added before autoclaving, but not both.
 
Deleted:
<
<		Ferrous Sulfate may be better added as 0.5ml of a 0.1% solution.

It might be best to autoclave the calcium chloride separate from the sulfates, as there seems to be a risk of calcium sulfate precipitate otherwise.

 
Final composition
190.2 mM Sodium (Na+)
11.0 mM Potassium (K+)
37.8 mM Ammonium (NH4)
0.09 mM Calcium (Ca2+)
0.83 mM Magnesium (Mg2+)
Changed:
<
<
38.0 mM Chloride (Cl-
>
>
38.0 mM Chloride (Cl-)
 
0.87 mM Sulfate (SO42-)
106.1 mM Phosphate (PO43-)
50.0 mM Lactic Acid

-- AurkoDasgupta - 03 Jun 2013

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