Difference: ProtocolsRecipesS2 (3 vs. 4)

Revision 42013-06-25 - AurkoDasgupta

 

S2

Used for Acinetobacter

1L 5L Component
1.5g 7.5 g KH2PO4
25.5g 127.5 g Na2HPO4 7H2O
0.1g 0.5 g MgSO4
2g 10 g NH4Cl
0.01g 0.05 g CaCl2
0.0005g 0.0025 g FeSO4 7H2O

To each 1L, add 5ml of 85-90% lactic acid. Bring final pH to 6.65.

Calcium Chloride may be better added as 1ml of 1% solution, or 90ul of 1M solution.

Ferrous Sulfate may be better added as 0.5ml of a 0.1% solution.

Added:
>
>		It might be best to autoclave the calcium chloride separate from the sulfates, as there seems to be a risk of calcium sulfate precipitate otherwise.
 
Final composition
Changed:
<
<
95.1 mM Sodium (Na+)
>
>
190.2 mM Sodium (Na+)
 
11.0 mM Potassium (K+)
37.8 mM Ammonium (NH4)
0.09 mM Calcium (Ca2+)
0.83 mM Magnesium (Mg2+)
38.0 mM Chloride (Cl-
0.87 mM Sulfate (SO42-)
106.1 mM Phosphate (PO43-)
50.0 mM Lactic Acid

-- AurkoDasgupta - 03 Jun 2013

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