Difference:
ProtocolsRecipesS2
(2 vs. 3)
Revision 3
2013-06-21 -
AurkoDasgupta
S2
Used for Acinetobacter
1L
5L
Component
1.5g
7.5 g
KH
2
PO
4
25.5g
127.5 g
Na
2
HPO
4
7H
2
O
0.1g
0.5 g
MgSO
4
2g
10 g
NH
4
Cl
0.01g
0.05 g
CaCl
2
0.0005g
0.0025 g
FeSO
4
7H
2
O
To each 1L, add 5ml of 85-90% lactic acid. Bring final pH to 6.65.
Calcium Chloride may be better added as 1ml of 1% solution, or 90ul of 1M solution.
Ferrous Sulfate may be better added as 0.5ml of a 0.1% solution.
Added:
>
>
Final composition
95.1 mM
Sodium (Na
+
)
11.0 mM
Potassium (K
+
)
37.8 mM
Ammonium (NH
4
)
0.09 mM
Calcium (Ca
2+
)
0.83 mM
Magnesium (Mg
2+
)
38.0 mM
Chloride (Cl
-
0.87 mM
Sulfate (SO
4
2-
)
106.1 mM
Phosphate (PO
4
3-
)
50.0 mM
Lactic Acid
-- Main.AurkoDasgupta - 03 Jun 2013
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