Difference: ProtocolsRecipesMinimalGlucose (1 vs. 12)

Revision 122025-01-09 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when agar and phosphate or phosphate and glucose are autoclaved together.
Changed:
<
<
500 ml 1L 1.5L Component MW
2.65 g 5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
166.7 ml 333 ml 500 ml dH2O  
>
>
500 mL 1.0L Component
2.65 g 5.3 g Potassium Phosphate (dibasic) K2HPO4
1 g 2 g Potassium Phosphate (monobasic) KH2PO4
0.5 g 1 g Ammonium Sulfate (NH4)2 SO4
0.25 g 0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2
166.7 mL 333 mL dH2O
  Separately, prepare the agar:
Changed:
<
<
500 ml 1L 1.5L Component
8 g 16 g 24 g Agar
500 µl 1 ml 1.5 ml Antifoam (5%)
166.7 ml 333 ml 500 ml dH2O
>
>
500 mL 1.0 L Component
8 g 16 g Agar
500 µL 1 mL Antifoam (5%)
166.7 mL 333 mL dH2O
Deleted:
<
<
Autoclave.
  Next, prepare the sugar solution. The final concentration is 0.4% w/v:
Changed:
<
<
500 ml 1L 1.5L Component
2 g 4 g 6 g Glucose, arabinose, or other sugar
166.7 ml 333 ml 500 ml dH2O
Autoclave.
>
>
500 mL 1.0 L Component
2 g 4 g Glucose, arabinose, or other sugar
166.7 mL 333 mL dH2O
Autoclave the 3 parts separately for a 40 minute sterilization time.
  After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:
Changed:
<
<
1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)
>
>
500 mL 1.0 L Component
500 µL 1.0 ml 10% MgSO4 (separately autoclaved stock)
500 µL 1.0 ml 0.2% Thiamine (vitamin B1) (filter sterilized stock refrigerated at 4ºC)
  Source: Lenski Lab Protocol

Revision 112024-10-22 - AlexaMorton

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when agar and phosphate or phosphate and glucose are autoclaved together.
Changed:
<
<
1L 1.5L Component MW
5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
>
>
500 ml 1L 1.5L Component MW
2.65 g 5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
1 g 2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
0.5 g 1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.25 g 0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
166.7 ml 333 ml 500 ml dH2O  
Deleted:
<
<
Autoclave.
 
Changed:
<
<
Next, prepare the agar:
>
>
Separately, prepare the agar:
 
Changed:
<
<
1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.
>
>
500 ml 1L 1.5L Component
8 g 16 g 24 g Agar
500 µl 1 ml 1.5 ml Antifoam (5%)
166.7 ml 333 ml 500 ml dH2O
Autoclave.
 
Changed:
<
<
Next, prepare the sugar. The final concentration is 0.4% w/v:
>
>
Next, prepare the sugar solution. The final concentration is 0.4% w/v:
 
Changed:
<
<
1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.
>
>
500 ml 1L 1.5L Component
2 g 4 g 6 g Glucose, arabinose, or other sugar
166.7 ml 333 ml 500 ml dH2O
Autoclave.
  After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)

Source: Lenski Lab Protocol

Revision 102020-09-26 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when agar and phosphate or phosphate and glucose are autoclaved together.

1L 1.5L Component MW
5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.
Changed:
<
<
Next, prepare the sugar (0.2%):
>
>
Next, prepare the sugar. The final concentration is 0.4% w/v:
 
1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)

Source: Lenski Lab Protocol

Revision 92015-02-04 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Changed:
<
<
Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately.
>
>
Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately. Compounds that inhibit growth are produced when agar and phosphate or phosphate and glucose are autoclaved together.
 
1L 1.5L Component MW
5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)

Source: Lenski Lab Protocol

Revision 82013-02-19 - AurkoDasgupta

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately.

1L 1.5L Component MW
Changed:
<
<
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
>
>
5.3 g 8 g Potassium Phosphate (dibasic) K2HPO4 MW 174.18
2 g 3 g Potassium Phosphate (monobasic) KH2PO4 MW 136.07
 
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)

Source: Lenski Lab Protocol

Revision 72012-10-28 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"
Changed:
<
<

MG: Minimal Glucose, aka DM: Davis Minimal

>
>

MG: Minimal Glucose agar, aka DM: Davis Mingioli agar

 Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately.

1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.
Changed:
<
<
After the 3 parts have been autoclaved, combine the contents of the three flasks together and add the following stock solutions:
>
>
After the 3 parts have been autoclaved, combine the contents of the three flasks together while they are still warm add the following stock solutions:
 
1L 1.5L Component
Changed:
<
<
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (autoclaved)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)
>
>
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (separately autoclaved stock)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized stock)
Deleted:
<
<
  Source: Lenski Lab Protocol

Revision 62012-05-04 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose, aka DM: Davis Minimal

Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately.

1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together and add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (autoclaved)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)
Added:
>
>
Source: Lenski Lab Protocol
 

Revision 52012-05-02 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose, aka DM: Davis Minimal

Changed:
<
<
>
>
Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar) separately.
Deleted:
<
<
Note: Salts, agar and sugars need to be prepared and autoclaved separately
Also note: When making these plates it is necessary to prepare the 3 main parts (salts, agar, and sugar) separately . Each must be prepared and autoclaved independent of the others. Please follow the order below.
 
1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After the 3 parts have been autoclaved, combine the contents of the three flasks together and add the following stock solutions:

1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (autoclaved)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)

Revision 42011-11-30 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose, aka DM: Davis Minimal

Changed:
<
<
Note: Salts, agar and sugars need to be prepared and autoclaved separately
>
>
Note: Salts, agar and sugars need to be prepared and autoclaved separately
Added:
>
>
Also note: When making these plates it is necessary to prepare the 3 main parts (salts, agar, and sugar) separately . Each must be prepared and autoclaved independent of the others. Please follow the order below.
 
1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.
Changed:
<
<
After autoclaving combine the contents of the three flasks together and add the following stock solutions:
>
>
After the 3 parts have been autoclaved, combine the contents of the three flasks together and add the following stock solutions:
 
1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (autoclaved)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)

Revision 32011-10-05 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose, aka DM: Davis Minimal

Changed:
<
<
Notes: Salts, agar and sugars need to be prepared and autoclaved separately.
>
>
Note: Salts, agar and sugars need to be prepared and autoclaved separately
 
1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar (0.2%):

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After autoclaving combine the contents of the three flasks together and add the following stock solutions:

Changed:
<
<
1L 1.5L Component MW
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) Note Thiamin must be syringe filtered into the media
>
>
1L 1.5L Component
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 (autoclaved)
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) (filter sterilized)
 

Revision 22011-08-03 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"
Added:
>
>
 

MG: Minimal Glucose, aka DM: Davis Minimal

Notes: Salts, agar and sugars need to be prepared and autoclaved separately.

1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.
Changed:
<
<
Next, prepare the sugar:
>
>
Next, prepare the sugar (0.2%):
 
1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After autoclaving combine the contents of the three flasks together and add the following stock solutions:

1L 1.5L Component MW
Changed:
<
<
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  
>
>
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) Note Thiamin must be syringe filtered into the media
 

Revision 12011-07-29 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

MG: Minimal Glucose, aka DM: Davis Minimal

Notes: Salts, agar and sugars need to be prepared and autoclaved separately.

1L 1.5L Component MW
7 g 10.5 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
2 g 3 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g 1.5 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g 0.75 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
333 ml 500 ml dH2O  
Autoclave.

Next, prepare the agar:

1L 1.5L Component
16 g 24 g Agar
1 ml 1.5 ml Antifoam (5%)
333 ml 500 ml dH2O
Autoclave.

Next, prepare the sugar:

1L 1.5L Component
4 g 6 g Glucose (or other sugar)
333 ml 500 ml dH2O
Autoclave.

After autoclaving combine the contents of the three flasks together and add the following stock solutions:

1L 1.5L Component MW
1.0 ml 1.5 ml 10% Magnesium Sulfate MgSO4 MW 120.37  
1.0 ml 1.5 ml 0.2% Thiamin (vitamin B1) Note* Thiamin must be syringe filtered into the media  

 
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