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META TOPICPARENT |
name="ProtocolsMediaRecipes" |
M9 Minimal Media Plates |
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> > | As with DM and MG media, make sure to autoclave the agar and phosphate separately. |
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For 1 liter of media:
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6 g |
Sodium phosphate, Na2HPO4 (anhydrous) |
3 g |
Potassium phosphate, KH2PO4 |
0.5 g |
Sodium chloride, NaCl |
1 g |
Ammonium chloride, NH4Cl |
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Add dH2O to 500 mL
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< < | Add dH2O to 1 liter. Autoclave. |
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< < | After media is autoclaved add the following sterile ingredients:
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> > | Add dH2O to 500 mL. |
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> > | Autoclave media. Once the bottles are cooled to 55°C, mix the agar and M9 components.
Next, add the following sterile ingredients:
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1 ml |
1M Magnesium sulfate, MgSO4 |
1 ml |
0.1M Calcium chloride CaCl2 |
10 ml |
10% (w/v) glucose (or other carbon source) |
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