Difference: ProtocolsRecipesLysogenyBrothAgar (8 vs. 9)

Revision 92024-09-05 - SophiaLin

 
META TOPICPARENT name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Changed:
<
<		Combine in a 2 L flask the following:
>
>		Add dH2O to full volume in an appropriately-sized flask. Combine the following, making sure each component fully dissolves before adding the next:
 
Changed:
<
<
1.5L Component
15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam
>
>
1L 6L Component
10 g 60 g Tryptone
5 g 30 g Yeast Extract
10 g 60 g NaCl
1 ml 6 ml 5% Antifoam*
16 g 48 g Agar*
 
Changed:
<
<		Add dH2O to 1.5 L. Autoclave.
>
>		ANTIFOAM: Open the bottle under sterile conditions (near a flame) only, and continue agitating after the addition of the 5% antifoam.
Added:
>
>		 AGAR: Measure 8 grams of agar per 500 ml bottle and add to each individual bottle before aliquoting the LB. Autoclave to homogenize.

Each liter of LB Agar makes 2 bottles of 500 ml.

  Notes and variations:
  • Common synonyms are Luria-Broth and Luria-Bertani medium.
  • This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.
  • If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
  • To make "soft" or "top" agar for phage studies, use only 7-8 g agar per liter.

META TOPICMOVED by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"
View topic | History: r11 < r10 < r9 < r8 | More topic actions...
 
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