Difference: ProtocolsRecipesLysogenyBrothAgar (6 vs. 7)

Revision 72019-06-06 - JeffreyBarrick

META TOPICPARENT	name="ProtocolsMediaRecipes"

LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

Combine in a 2 L flask the following:

1.5L	Component
15 g	Tryptone
7.5 g	Yeast Extract
15 g	NaCl
24 g	Agar
1.5 ml	5% Antifoam

Add dH₂O to 1.5 L. Autoclave.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.

Changed:

<
<

This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter! They are NOT interchangeable.

>
>

This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.

If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
To make "soft" agar for phage studies, use only 7 g agar per liter.

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"

View topic | History: r11 < r10 < r9 < r8 | More topic actions...

Copyright © 2008-2025 by the contributing authors. All material on this collaboration platform is the property of the contributing authors.
Ideas, requests, problems regarding TWiki? Send feedback