Difference: ProtocolsRecipesLysogenyBrothAgar (5 vs. 6)

Revision 62015-06-23 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"
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LB Agar: Lysogeny Broth

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LB: Lysogeny Broth / Luria-Bertani (Miller) Agar

  Combine in a 2 L flask the following:

1.5L Component
15 g Tryptone
7.5 g Yeast Extract
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15 g NaCl
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15 g NaCl
 
24 g Agar
1.5 ml 5% Antifoam

Add dH2O to 1.5 L. Autoclave.

Notes and variations:

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  • This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter!*
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  • This is the "Miller" formulation of LB. Another common formulation has only 5 g NaCl per liter! They are NOT interchangeable.
 
  • If you are performing sacB counter-selection, you may need to use even lower salt concentrations.
  • To make "soft" agar for phage studies, use only 7 g agar per liter.
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META TOPICMOVED by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"
 
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