Difference: ProtocolsRecipesLysogenyBrothAgar (9 vs. 10)

META TOPICPARENT	name="ProtocolsMediaRecipes"

Add dH₂O to full volume in an appropriately-sized flask. Combine the following, making sure each component fully dissolves before adding the next:

ANTIFOAM: Open the bottle under sterile conditions (near a flame) only, and continue agitating after the addition of the 5% antifoam.

AGAR: Measure 8 grams of agar per 500 ml bottle and add to each individual bottle before aliquoting the LB. Autoclave to homogenize.

Each liter of LB Agar makes 2 bottles of 500 ml.

Notes and variations:

Common synonyms are Luria-Broth and Luria-Bertani medium.
This is the "Miller" formulation of LB with 10 g NaCl per liter (1% w/v). Another common formulation has only 5 g NaCl per liter (0.5% w/v)! They are NOT interchangeable.

Changed:

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If you are performing sacB counter-selection, you may need to use even lower salt concentrations.

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If you are performing sacB counter-selection, you do not want to include NaCl in your agar. Use the above recipe, but swap out 5-10% w/v (50-100g per liter) sucrose in place of any NaCl. Sucrose can be autoclaved (no need to filter sterilize it separately).

META TOPICMOVED	by="JeffreyBarrick" date="1435069038" from="Lab.ProtocolsRecipesLysogenyBroth" to="Lab.ProtocolsRecipesLysogenyBrothAgar"