topA Gene Sequencing

The topA gene coordinates are 1329420-1332017.

The topA mutation in the Ara-1 long-term line is 1329516:C→T.

primer <-- -->	who	coords	sequence
RL0398	TC	1329010-1329031	5'‑CGCGGTCGATGGGTTGTGTCTC
RL0399	TC	1332121-1332101	5'‑ATCATTTATAGCCTTAGTGTA
RL0400	TC	1329557-1329577	5'‑GAGTGCCGACTCTACCTCCAC
RL0401	TC	1330088-1330109	5'‑TGCCAGCACGACCACGCCATCT
RL0402	TC	1330609-1330629	5'‑AGTTCGTTGCCTGCCAGATGA
RL0403	TC	1331103-1331122	5'‑GCTGTACTGGATCACTTCTT
RL0410, RL0610	RW, BW	1329489-1329508	5'‑GACTACGTGGTGAAATCCAG
RL0411, RL0611	RW, BW	1329683-1329663	5'‑ACCAGGCAACACTTCATAGTG
RL0738	CO	1329010-1329029	5'‑CGCGGTCGATGGGTTGTGTC
RL0739	CO	1332323-1332302	5'‑TCCCGGGTTGTGATGTGTAAAG
RL0740	CO	1329648-1329670	5'‑CACAATTGGGAGGCGCACTATGA
RL0741	CO	1331694-1331672	5'‑GTAATGGCACCGGATCTTCTTTC
RL0742	CO	1329990-1330007	5'‑GCCTGTCTGCCGGTCGTGTG
RL0743	CO	1331055-1331032*	5'‑TCTGCGCGGTAAAgTCGTAGTTCA

* This primer has a base mismatch (lowercase) to REL606. It must have been designed based on the MG1655 sequence.

All coordinates are for the reference strain REL606.