Revision 42025-06-17 - AlexaMorton

  META TOPICPARENT 
 name="GoldenGateAssembly" 

 Generating Overhangs
- META TOPICPARENT
+ name="GoldenGateAssembly"
-<
<
+If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set.
->
>
+If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set.
-<
<
+For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly.
->
>
+For scarless assembly NEB's SPLITSET tool may be used. You'll need to input the DNA sequence you're assembling and specify the number of assembly fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly.
->
>
+These tools can be useful for domesticating part plasmids, for creating high-fidelity subparts for an existing assembly schema (such as the BTK/YTK), and for creating scarless assemblies.
-<
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+-- 
      No permission to view TWiki.UserReports
-<
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+ Comments
->
>
+--  
 Warning
 Can't INCLUDE TWiki.UserReports repeatedly, topic is already included.  
 
 
 
-- Updated by 
      No permission to view TWiki.UserReports
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+No such template def TMPL:DEF{PROMPT:above}

Revision 32021-11-04 - VictorLi

META TOPICPARENT	name="GoldenGateAssembly"

Generating Overhangs

If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set.

For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly.

--

Warning: Can't INCLUDE TWiki.UserReports repeatedly, topic is already included.

Comments

No such template def TMPL:DEF{PROMPT:above}

Revision 22021-11-03 - VictorLi

META TOPICPARENT	name="GoldenGateAssembly"

Generating Overhangs

Added:

>
>

If generating overhangs for Golden Gate Assembly outside of YTK/BTK framework, NEB's GETSET tool can be useful for generating a set of high fidelity overhangs to use. If compatibility with other overhangs is necessary required overhangs may be set.

For scarless assembly NEB's SPLITSET tool may be used. You'll need to specify the DNA sequence you're assembling, and how it will be broken up into fragments. The tool will find high fidelity overhangs that will allow you to scarlessly assembly your DNA using Golden Gate Assembly.

--

Warning: Can't INCLUDE TWiki.UserReports repeatedly, topic is already included.

Comments

No such template def TMPL:DEF{PROMPT:above}

Revision 12021-11-03 - VictorLi

META TOPICPARENT	name="GoldenGateAssembly"

Generating Overhangs

--

Warning: Can't INCLUDE TWiki.UserReports repeatedly, topic is already included.

Comments

No such template def TMPL:DEF{PROMPT:above}

Difference: GeneratingOverhangs (1 vs. 4)

Revision 42025-06-17 - AlexaMorton

Generating Overhangs

Comments

Revision 32021-11-04 - VictorLi

Generating Overhangs

Comments

Revision 22021-11-03 - VictorLi

Generating Overhangs

Comments

Revision 12021-11-03 - VictorLi

Generating Overhangs

Comments