Commonly Used Plasmids

plasmid markers origin ref description
pCA24N CamR   Kitagawa2005 ASKA collection vector
pKD46 AmpR TS Datsenko&Wanner2000 Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD3 CamR   Datsenko&Wanner2000 Wanner template plasmid
pKD13 KanR   Datsenko&Wanner2000 Wanner template plasmid
pCR2.1 KanR, AmpR     TOPO cloning kit vector
pACYC184 TetR, CamR   Chang&Cohen1978 Lenski evolution experiment
pKO3, pKOV SucS, CamR   Link1997 Church vectors for creating deletions
pRA300, pRA301, pRA302 SpecR   Akakura2002 Broad host range, promoterless lacZ map

Common Plasmid Box

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid strain
pACBSR TOP10
pKD3 BW25141
pKD13 BW2514
pKD32 BW2514
pKD46 BW25141
pCP20 BT340
pRA300 ?
pRA301 ?
pRA302 ?
pACYC184 ER2420

References

Akakura2002 Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978 Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000 Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005 Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997 Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.
Attachments Attachments
Topic attachments
I Attachment History Action Size Date Who Comment
Unknown file formatgbk pACYC184.gbk r1 manage 7.7 K 2009-08-03 - 21:09 JeffreyBarrick  
Unknown file formatgbk pCA24N.gbk r1 manage 9.9 K 2009-05-29 - 13:21 JeffreyBarrick  
Unknown file formatgbk pCR2.1.gbk r2 r1 manage 7.1 K 2012-03-07 - 21:11 JeffreyBarrick  
Unknown file formatgbk pKD13_Kan_template.gbk r1 manage 7.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgbk pKD3_Cam_template.gbk r1 manage 6.6 K 2009-05-29 - 13:22 JeffreyBarrick  
Unknown file formatgb pKD46.gb r1 manage 16.4 K 2012-01-20 - 21:09 BrianRenda  
Texttxt pKOV.txt r1 manage 10.6 K 2011-02-21 - 17:13 JeffreyBarrick  
Unknown file formatgbk pkd4.gbk r1 manage 6.1 K 2011-12-19 - 22:29 BrianRenda  
Unknown file formatgbk pkd4_with_gfp_sites_marked.gbk r1 manage 10.0 K 2011-12-19 - 22:35 BrianRenda  
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Contributors to this topic Edit topic JeffreyBarrick, CraigBarnhart, DanielDeatherage, BrianRenda, AlvaroRodriguez
Topic revision: r17 - 2012-07-17 - 20:07:54 - Main.AlvaroRodriguez
 
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