UV mutagenesis of E. coli

Stratagene UV Crosslinker (located in the Schmidt lab). Prewarm the machine by running it for 2 minutes. Then set the machine for "250". The units on this number are 100 J / m2. This amount of radiation kills 99.9% of the cells for Escherichia coli strain REL606.

A typical colony on a plate has roughtly 5-50 million cells.

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Topic revision: r3 - 17 Feb 2011 - 15:54:23 - JeffreyBarrick
 
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